K2217-100 assays | Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric

Background

α-Ketoglutarate Dehydrogenase (α-KGDH) is an important enzyme in the citric acid cycle. Combining with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3), α-KGDH forms an enzyme complex. In the presence of NAD⁺ and CoA, α-KGDH converts α-ketoglutarate into succinyl-CoA. α-KGDH is highly regulated by intracellular calcium, NADH/NAD⁺ and ATP/ADP ratios. Decreased α-KGDH activity can lead to Alzheimer’s disease. α-KGDH is also a target of oxidative stress. Reactive oxygen species (ROS) can inhibit α-KGDH activity which then diminishes its important function and causes a bioenergetic deficit.

The Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection α-KGDH activity in various samples based on colorimetric method. In the assay, α-KGDH changes α-ketoglutarate to an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The kit can detect α-KGDH activity lower than 0.1 mU in a variety of samples.

Features & Properties

Features	Simple, rapid & convenient; Can measure α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

KGDH Assay Buffer, KGDH Substrate (Lyophilized), KGDH Developer (Lyophilized), NADH Standard (Lyophilized), KGDH Positive Control

Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit

Referência K2217-100 assays

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