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> Anti-Cyclooxygenase 1 Polyclonal Antibody 3

Anti-Cyclooxygenase 1 Polyclonal Antibody 3

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Referência NB-64-154803-200uL

Tamanho : 200uL

Marca : Neo Biotech


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Anti-Cyclooxygenase 1 Polyclonal Antibody 3

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Catalog No. TMAB-04847 Copy Product Info
Anti-Cyclooxygenase 1 Polyclonal Antibody 3 is a Rabbit antibody targeting Cyclooxygenase 1. Anti-Cyclooxygenase 1 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Cyclooxygenase 1 Polyclonal Antibody 3 is a Rabbit antibody targeting Cyclooxygenase 1. Anti-Cyclooxygenase 1 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Pig,Cow,Sheep)
Verified Activity
1. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
A431 (Human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
Huvec (Human) Cell Lysate at 30 μg
Primary: Anti-Cyclooxygenase 1 (TMAB-04847) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
2. Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-COX-1 Polyclonal Antibody, Unconjugated (TMAB-04847) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMicrosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cyclooxygenase 1
Antigen Species
Human
Gene Name
PTGS1
Gene ID
5742
Protein Name
Prostaglandin G/H synthase 1
Uniprot ID
P23219
Function
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
Chemical Properties
Molecular WeightTheoretical: 63 kDa. Actual: 63 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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