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> Anti-EPO antibody

Anti-EPO antibody

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Referência NB-22-22194-50

Tamanho : 50μL

Marca : Neo Biotech

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Datasheet

  • Western blot analysis of extracts of various cell lines, using EPO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using EPO antibody (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using EPO antibody (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using EPO antibody (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse liver cells using EPO rabbit polyclonal antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

General Info

Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPO (93-193) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: EPO
Gene ID: 2056
Uniprot ID: EPO_HUMAN
Immunogen Region: 93-193
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 93-193 of human EPO (NP_000790.2).
Immunogen Sequence: GLALLSEAVLRGQALLVNSS QPWEPLQLHVDKAVSGLRSL TTLLRALGAQKEAISPPDAA SAAPLRTITADTFRKLFRVY SNFLRGKLKLYTGEACRTGD R

Description

Tissue Specificity Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Function Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
Protein Name Erythropoietin
Epoetin
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-9006335
Reactome: R-HSA-9027276
Reactome: R-HSA-9027277
Reactome: R-HSA-9027283
Reactome: R-HSA-9027284
Cellular Localisation Secreted
Alternative Antibody Names Anti-Erythropoietin antibody
Anti-Epoetin antibody
Anti-EPO antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Referência
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NB-22-22194
Anti-EPO antibody
 100μL