Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Anti-Fibrillin 1 Antibody

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Referência CPA4640-100ul

Tamanho : 100ul

Marca : Cohesion Biosciences

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Western blot analysis of Fibrillin 1 expression in mouse muscle (A) whole cell lysates. (Predicted band size: 312 kD; Observed band size: 310 kD)
Immunohistochemical analysis of Fibrillin 1 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Fibrillin 1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-Fibrillin 1 Antibody
Cat No:	CPA4640
Source:	Rabbit
Reactivity:	H, M, R, B, P
Applications:	WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to Fibrillin 1

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Fibrillin 1. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of Fibrillin 1 protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene Symbol：FBN1

Alternative Names：FBN; Fibrillin-1

Entrez Gene (Human)： 2200;

SwissProt (Human)： P35555;

SwissProt (Mouse)： Q61554;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.