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> Anti-GPR105 Antibody

Anti-GPR105 Antibody

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Referência CPA2438-30ul

Tamanho : 30ul

Marca : Cohesion Biosciences


  • Western blot analysis of GPR105 expression in HEK293T (A), mouse heart (B), rat heart (C) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 45 kD)
  • Immunohistochemical analysis of GPR105 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of GPR105 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Product Name:Anti-GPR105 Antibody
Cat No:CPA2438
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to GPR105
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR105. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of GPR105 protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)
Gene Symbol：P2RY14
Alternative Names：GPR105; KIAA0001; P2Y purinoceptor 14; P2Y14; G-protein coupled receptor 105; UDP-glucose receptor
Entrez Gene (Human)： 9934;
Entrez Gene (Mouse)： 140795;
Entrez Gene (Rat)： 171108;
SwissProt (Human)： Q15391;
SwissProt (Mouse)： Q9ESG6;
SwissProt (Rat)： O35881;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.