Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.
 
> Anti-PRDM14 Antibody

Anti-PRDM14 Antibody

Imprimir Imprimir
Please log in

Referência CQA1229-50ul

Tamanho : 50ul

Marca : Cohesion Biosciences


  • Western blot analysis of PRDM14 expression in BT474 (A), Jurkat (B), Hela (C) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 70 kD)
  • Immunohistochemical analysis of PRDM14 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of PRDM14 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Product Name:Anti-PRDM14 Antibody
Cat No:CQA1229
Source:Rabbit
Reactivity:H, M
Applications:WB, IH, IF/IC, IP, ChIP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to PRDM14
Immunogen：Recombinant full length protein of human PRDM14
Purification：The antibody was purified by immunogen affinity chromatography.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/20 - 1/100), ChIP (1/20 - 1/100)
Gene Symbol：PRDM14
Alternative Names：PR domain zinc finger protein 14; PR domain-containing protein 14
Entrez Gene (Human)： 63978;
Entrez Gene (Mouse)： 383491;
SwissProt (Human)： Q9GZV8;
SwissProt (Mouse)： E9Q3T6;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.