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Anti-TLR4 Antibody (1M331)

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Referência NB-64-163489-50uL

Tamanho : 50uL

Marca : Neo Biotech


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Anti-TLR4 Antibody (1M331)

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Synonyms:

Catalog No. TMAB-13571 Copy Product Info
Anti-TLR4 Antibody (1M331) is a Mouse antibody targeting TLR4. Anti-TLR4 Antibody (1M331) can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-TLR4 Antibody (1M331) is a Mouse antibody targeting TLR4. Anti-TLR4 Antibody (1M331) can be used in WB.
Ig Type
IgG1, k
Clone
1M331
Reactivity
Mouse,Human,Rat
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with TLR4 monoclonal antibody, unconjugated (TMAB-13571) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. THP-1 (H) cells were treated with or without TPA (80 nM) for 18 h, incubated in fresh medium for 48 h, and LPS (5 μg/mL) for 18 h, 25 μg total protein per lane of cell lysates (see on figure) probed with TLR4 monoclonal antibody, unconjugated (TMAB-13571) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
WB
Recommended Dose
WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
Tissue SpecificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe Toll-like receptor 4 by activating natural immunity, specific immune response involved in the start-up, Toll-like receptor 4 as an important signal transduction transmembrane receptor involved in the toxin-induced inflammation in the pathological process, its mechanisms of control On a growing concern. all regions were either double-stranded or sequenced with an alternatechemistry or covered by high quality data(i.e., phred quality >=30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by at least one subclone; and the assembly was confirmed by restriction digest, except on the rare occasion of the clone being a YAC. The following abbreviations are used to associate primary accession numbers given in the feature table with their source databases: Em:, EMBL; Sw:, SWISSPROT; Tr:, TREMBL; Wp:, WORMPEP; Information on the WORMPEP database can be found at. TLR-4 plays an important role in microvascular leakage and leukocyte adhesion under the inflammatory condition associated with nonseptic thermal injury.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse TLR4
Antigen Species
Mouse
Gene Name
TLR4
Gene ID
21898
Protein Name
Toll-like receptor 4
Uniprot ID
Q9QUK6
Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
Chemical Properties
Molecular WeightTheoretical: 90 kDa. Actual: 100 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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