Anti-XCR1 [MARX10]
Referência MBS489103-0,2mg
Tamanho : 0.2mg
Marca : MyBiosource
Immunofluorescence staining of mouse splenocytes with anti-XCR1 antibody MARX10 . Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MARX10 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
XCR1 Antibody
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS489103The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 × 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis.XCR1 expression determines Batf3-dependent… More details
Product Overview
Online Datasheet in PDFProduct Name
XCR1, Antibody
Full Product Name
Anti-XCR1 [MARX10]
Product Gene Name
anti-XCR1 antibody
Product Synonym Names
mXCR1; Ccxcr1; Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; MARX1O
Isotype
Mouse IgG2b kappa
Clone Number
MARX10
Species Reactivity
Mouse, Rat
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Purified. Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
37,308 Da
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Product Categories/Family for anti-XCR1 antibody
signal transduction; chemokine binding; calcium-mediated signaling; chemotaxis
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-XCR1 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), in vivo
Application Notes for anti-XCR1 antibody
This antibody was used to stain cryostat sections (12 um) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20% of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012; PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1+ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay; MARX10-OVA was found to be highly effective in inducing CD8+ T cell cytotoxicity in vivo (Hartung et al., 2015; PMID: 25520399).
Immunofluorescence (IF) of anti-XCR1 antibody
Immunofluorescence staining of mouse splenocytes with anti-XCR1 antibody MARX10 . Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MARX10 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Product References and Citations for anti-XCR1 antibody
Bachem et al.
Front Immunol. 2012 Jul 18;3:214.
Front Immunol. 2012 Jul 18;3:214.
Other Notes
Small volumes of anti-XCR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for XCR1 purchase
MBS489103 is an antibody targeting XCR1. Also known as Anti-XCR1 [MARX10]. Reactive in Mouse, Rat.
Additional Details
NCBI/Uniprot data below describe general gene information for XCR1. It may not necessarily be applicable to this product.
NCBI Official Full Name
Chemokine XC receptor 1
NCBI Official Synonym Full Names
chemokine (C motif) receptor 1
NCBI Official Symbol
Xcr1
NCBI Official Synonym Symbols
Gpr5; mXcr1; Ccxcr1
UniProt Protein Name
Chemokine XC receptor 1
UniProt Synonym Protein Names
Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; mXCR1
Protein Family
Chemokine XC receptor
UniProt Gene Name
Xcr1
UniProt Synonym Gene Names
Ccxcr1
UniProt Entry Name
XCR1_MOUSE
NCBI Protein Information
chemokine XC receptor 1; SCM1 receptor; lymphotactin receptor; XC chemokine receptor 1; C motif-1/lymphotactin receptor; chemokine (C motif) XC receptor 1
UniProt Comments for XCR1
XCR1: Receptor for chemokines SCYC1 and SCYC2. Subsequently transduces a signal by increasing the intracellular calcium ions level. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis
Cellular Component: cell; intracellular; cytosol
Biological Process: release of sequestered calcium ion into cytosol; response to cytokine stimulus; chemotaxis
Show more Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis
Cellular Component: cell; intracellular; cytosol
Biological Process: release of sequestered calcium ion into cytosol; response to cytokine stimulus; chemotaxis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-XCR1 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Chemokine Receptors Bind Chemokines Pathway antibodies | Chemokine Receptors Bind Chemokines Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Class A/1 (Rhodopsin-like Receptors) Pathway antibodies | Class A/1 (Rhodopsin-like Receptors) Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
| G Alpha (q) Signalling Events Pathway antibodies | G Alpha (q) Signalling Events Pathway Diagram |
| GPCR Downstream Signaling Pathway antibodies | GPCR Downstream Signaling Pathway Diagram |
| GPCR Ligand Binding Pathway antibodies | GPCR Ligand Binding Pathway Diagram |
| GPCRs, Other Pathway antibodies | GPCRs, Other Pathway Diagram |
Organs/Tissues associated with anti-XCR1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Spleen Antibodies | >5 publications with XCR1 and Spleen |