Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.
 
> Antiviral agent 24 [23661-03-2]

Antiviral agent 24 [23661-03-2]

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Referência HY-151926-50mg

Tamanho : 50mg

Marca : MedChemExpress


Description

Antiviral agent 24 is a potent antiviral agent with EC50 values of 0.101, 19.9, 91.2 µM for EV71, CVA21, EV68, respectively. Antiviral agent 24 inhibits METTL3/METTL14 activity in a dose-dependent manner[1].

Cellular Effect
Cell Line Type Value Description References
G-361 IC50
> 166.7 μM
Compound: 44
Antitumor activity against human G361 cells after 72 hrs by Calcein AM assay
Antitumor activity against human G361 cells after 72 hrs by Calcein AM assay
[PMID: 17418578]
HOS IC50
> 166.7 μM
Compound: 44
Antitumor activity against human HOS cells after 72 hrs by Calcein AM assay
Antitumor activity against human HOS cells after 72 hrs by Calcein AM assay
[PMID: 17418578]
K562 IC50
> 166.7 μM
Compound: 44
Antitumor activity against human K562 cells after 72 hrs by Calcein AM assay
Antitumor activity against human K562 cells after 72 hrs by Calcein AM assay
[PMID: 17418578]
RD CC50
> 100 μM
Compound: 2; 11c
Cytotoxicity against human RD cells by MTS assay
Cytotoxicity against human RD cells by MTS assay
[PMID: 36455356]
RD CC50
> 235 μM
Compound: 8
Cytotoxicity against human RD cells
Cytotoxicity against human RD cells
[PMID: 38128296]
Vero CC50
> 50 μM
Compound: 8
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 7 days by resazurin staining based analysis
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 7 days by resazurin staining based analysis
[PMID: 38128296]
Vero C1008 CC50
> 100 μM
Compound: 2; 11c
Cytotoxicity against african green monkey Vero E6 cells by MTS assay
Cytotoxicity against african green monkey Vero E6 cells by MTS assay
[PMID: 36455356]
In Vitro

Antiviral agent 24 (compound 25) (0-1000 µM) inhibits METTL3/METTL14 complex activity with an EC50 value of 129 µM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Antiviral agent 24 Related Antibodies

Solvant et solubilité
In Vitro: 

DMSO : 250 mg/mL (587.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3509 mL 11.7547 mL 23.5095 mL
5 mM 0.4702 mL 2.3510 mL 4.7019 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Pureté et documentation
Références