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ASIC1 Antibody

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Referência ASM10261-A680-100

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  • ICC/IF - ASIC1 Antibody ASM10261
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ASIC1 Monoclonal Antibody, Clone S271-44 (ASM10261). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody (ASM10261) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ASIC1 Antibody (D) Composite.
  • WB - ASIC1 Antibody ASM10261
    Western Blot analysis of Rat brain lysates showing detection of ASIC1 protein using Mouse Anti-ASIC1 Monoclonal Antibody, Clone S271-44 (ASM10261). Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody (ASM10261) at 1:100, 1:250, 1:500, and 1:1000.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession Q6NXK8
Other Accession NP_033727.1
Host Mouse
Isotype IgG1
Reactivity Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Mouse ASIC1 Monoclonal IgG1
Target/Specificity Detects ~60kDa.
Other Names ACCN2 Antibody, Acid-sensing (proton-gated) ion channel Antibody, Acid-sensing ion channel 1 Antibody, Amiloride-sensitive cation channel 2 neuronal Antibody, BNAC2 Antibody, Brain sodium channel 2 Antibody
Clone Names S271-44
Immunogen Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-427 was sufficient for detection of ASIC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane