Aspartate Colorimetric/Fluorometric Assay Kit

Aspartate (Asp) is an α-amino acid. L-Aspartic acid is one of the 23 proteinogenic amino acids and serves as a precursor to four essential amino acids (Met, Thr, Ile and Lys). Aspartate is a metabolite in the urea cycle and is involved in gluconeogenesis and transports reducing equivalents between the mitochondria and the cytosol via the malate-aspartate shuttle. Also, aspartate is an excitotoxin and serves as an excitatory neurotransmitter in the brain through the stimulation of NMDA receptors.

The Aspartate Colorimetric/Fluorometric Assay Kit provides a sensitive, simple and convenient way for detection of aspartate in a variety of samples based on colorimetric and fluorometric method. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a fluorescent (Ex/Em 535/587 nm) and highly colored (570 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1-10 nmoles/well (2-200 µM).