RayBio® Bovine TNF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Bovine TNF-alpha ELISA Kit
Bovine TNF-alpha ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Bovine (cow) |
| Sensitivity | 0.1 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.1 ng/ml - 30 ng/ml |
| Specificity | This ELISA antibody pair detects bovine TNF-alpha. Other species not determined |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | Q06599 |
| Gene Id | 280943 |
| Gene Symbols | TNF, TNFA, TNFSF2 |
| Protein Name / Synonyms | Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Bovine TNF-alpha qIPCR ELISA Kit
As low as $779.00
Target Background
Tumor necrosis factor alpha (TNF alpha), also known as lymphotoxin and cachectin, is a pleiotropic cytokine. TNF alpha is a smaller cytokine with a molecular weight of 26 kDa (transmembrane) and 17 kDa (soluble) that binds to the same receptors producing a vast array of effects similar to those of TNF-beta. Both TNF-alpha and TNF-beta share 30% amino acid homology and have similar biological activities. TNF-alpha is produced primarily by monocytes, neutrophils and macrophages. It is found in synovial cells and macrophages in the tissues. TNF-alpha and TNF-beta can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 (55 kDa) and 2 (75 kDa) to be killed, with the p55 receptor mediating the cytotoxic response.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Bovine TNF-alpha ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified TNF alpha (TNFA), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Effects of gut barrier dysfunction during a viral respiratory disease challenge on immune function of feedlot beef calves
- Species:
- Bovine
- Sample Type:
- Serum (feedlot calves, BHV-1 challenge)
- Year:
- 2026
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 41968102
Feeding a Bacillus-based probiotic to beef heifers during stress challenges: effects on feed intake, immune, and metabolic responses
- Year:
- 2025
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 41335446
The profile of inflammatory factors in dairy calves with Cryptosporidium infection
- Species:
- Cow
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Veterinary Research Forum
Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)
- Species:
- Bovine
- Sample Type:
- sera
- Year:
- 2022
- Journal Name:
- Veterinary Research
Physiologic, health, and production responses of dairy cows supplemented with an immunomodulatory feed ingredient during the transition period
- Species:
- Bovine
- Sample Type:
- Serum (LPS challenged cows)
- Year:
- 2016
- Journal Name:
- Journal of Dairy Science
- PubMed ID:
- 27085398
Local and systemic inflammatory response to the intrauterine infusion of enzymes during estrus in water buffaloes with subclinical endometritis
- Species:
- Bovine
- Sample Type:
- uterine lavage, serum
- Year:
- 2023
- Journal Name:
- Research in Veterinary Science
DDX19A Senses Viral RNA and Mediates NLRP3-Dependent Inflammasome Activation
- Species:
- Porcine
- Sample Type:
- Conditioned Media (Primary pulmonary alveolar macrophages infected with porcine reproductive respt virus)
- Year:
- 2015
- Journal Name:
- The Journal of Immunology
- PubMed ID:
- 26538395
MicroRNA-26b Modulates the NF-B Pathway in Alveolar Macrophages by Regulating PTEN
- Species:
- Bovine
- Sample Type:
- Conditioned Media (Bovine alveolar macrophages treated with LPS of M. haemolytica LPS)
- Year:
- 2015
- Journal Name:
- Journal of Immunology
- PubMed ID:
- 26503952
Effects of oral meloxicam administration to beef cattle receiving lipopolysaccharide administration or vaccination against respiratory pathogens
- Species:
- Bovine
- Sample Type:
- Serum (LPS and vaccinated cattle testing)
- Year:
- 2015
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 26523594
Effects of vaccination against respiratory pathogens on feed intake, metabolic, and inflammatory responses in beef heifers
- Species:
- Bovine
- Sample Type:
- Serum (respiratory vaccine studies in beef heifers)
- Year:
- 2015
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 26440344
