Traditional cell culture is mainly based on 2D culture. With the deepening of cell research, scientists have found that 2D cultured cells are significantly different from cells under physiological conditions in vivo. In order to better simulate the growth of cells in the body, scientists are increasingly using 3D cell culture technology.

Cell Counting Kit-3D (CCK-3D) is convenient and sensitive for detecting cell proliferation/toxicity of 3D cells. It uses a reaction substrate of the WST series, which can be reduced by the abundant dehydrogenase in living cells in the presence of electron-coupled reagents and converted to the soluble, orange formazan in culture media. The amount of formazan produced by dehydrogenase is directly and linearly related to the number of viable cells.

This reagent is optimized for viability assays of a variety of cultured 3D spheroids, such as drop plates, ultra-low adsorption plates, Matrigel-coated and agarose-coated 3D spheroids. At the same time, this reagent is ready to use, making it ideal for high-throughput screenings.

Figure 1: Verification diagram of this product. Compared with Competitor B, the detection effect of this product is more sensitive