DACH1 (NM_080759) Human Tagged ORF Clone
Referência RG221505
Tamanho : 10ug
DACH1 (NM_080759) Human Tagged ORF Clone
- TrueORF®
SKU
RG221505
DACH1 (tGFP-tagged) - Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DACH1 |
| Synonyms | DACH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | Plasmid Map  |
| ACCN | NM_080759 |
| ORF Size | 2124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_080759.4 |
| RefSeq Size | 5240 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 1602 |
| UniProt ID | Q9UI36 |
| Cytogenetics | 13q21.33 |
| Domains | Ski_Sno |
| Protein Families | Transcription Factors |
| Summary | This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009 |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC221505 | DACH1 (Myc-DDK-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1 | 10 ug | | |
| RC221505L1 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC221505L2 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, mGFP tagged | 10 ug | | |
| RC221505L3 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC221505L4 | Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, mGFP tagged | 10 ug | | |
| SC120348 | DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1 | 10 ug | | |
| SC327980 | DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1) transcript variant 1 | 10 ug | | |
Plasmid Map for RG221505
Download sequence for
Cloning scheme for RG221505
Circular map for RG221505
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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