OCOA20513 | DISC1 Protein Lysate Clinisciences

Datasheets/Manuals	Printable datasheet for DISC1 Protein Lysate (OCOA20513)

Product Info
Predicted Species Reactivity	Homo sapiens
Product Format	Lyophilized
Host	HEK293T
Additional Information	Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::	Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and Storage	The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Tag	C-MYC/DDK
Description	DISC1 HEK293T cell transient overexpression lysate (as WB positive control)

Gene Symbol	DISC1
Gene Full Name	DISC1 scaffold protein
Alias Symbols	C1orf136;disrupted in schizophrenia 1 protein;SCZD9.
NCBI Gene Id	27185
Protein Name	C4P0D2, NM_001164550, NP_001158022
Description of Target	Disrupted in schizophrenia 1 isoform 45, disrupted in schizophrenia 1 protein isoform m [Homo sapiens], Homo sapiens DISC1 scaffold protein (DISC1), transcript variant m, mRNA
Uniprot ID	C4P0D2
Protein Accession #	https://www.ncbi.nlm.nih.gov/protein/NP_001158022
Nucleotide Accession #	https://www.ncbi.nlm.nih.gov/nuccore/NM_001164550
Molecular Weight	45.2 kDa

DISC1 Protein Lysate