DMGDH ELISA Kit (Human)
Referência OKEH02236
Tamanho : 96Wells
Marca : Aviva Systems Biology
DMGDH ELISA Kit (Human) (OKEH02236)
| Datasheets/Manuals | Printable datasheet for DMGDH ELISA Kit (Human) (OKEH02236) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology DMGDH ELISA Kit (Human) (OKEH02236) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DMGDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DMGDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DMGDH captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.0% (n = 20) Mean Inter-assay CV%: < 8.1% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 39 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Dimethylglycine dehydrogenase, mitochondrial | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | DMGDH |
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| Gene Full Name | dimethylglycine dehydrogenase |
| Alias Symbols | dimethylglycine dehydrogenase, mitochondrial, DMGDHD, ME2GLYDH. |
| NCBI Gene Id | 29958 |
| Protein Name | Dimethylglycine dehydrogenase, mitochondrial |
| Description of Target | Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro. |
| Uniprot ID | Q9UI17 |
| Protein Accession # | NP_037523.2 |
| Nucleotide Accession # | NM_013391.3 |
| Protein Size (# AA) | 866 |
| Molecular Weight | 97 kDa |