DTT
Referência 40400120-3
Tamanho : 25g
Marca : bioWORLD
Contactar o distribuidor local :
Telefone : +1 850 650 7790
RayPlex Human Inflammation Bead Array
This RayPlex® kit is a cytometric bead array to quantitatively measure 6 - 13 human inflammatory factors (see panel options) with a flow cytometer. The assay has been optimized for serum, plasma, and cell culture supernatant.
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Product Description
Specifications
| Size | 192 tests, 48 tests, 480 tests, 96 tests |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 6, 9, 13 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | Fluorescent Microbeads |
| Method Of Detection | Flow Cytometry |
| Design Principle | Sandwich-based |
| Research Area | Inflammation |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | 4°C |
Target Names
6-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha
9-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70
13-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70, GCSF, IL-13, IL-23 p19, MCP-1 (CCL2)
Product Features
- Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Application Notes
Suggested Applications
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- Lyophilized Protein Standard Mix
- Biotinylated Detection Antibody Cocktail
- 10X PE Conjugate
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
- RayBio® Serum/Plasma Diluent
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. !
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
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