A7021-10g | EDC.HCl [25952-53-8] Clinisciences

IC50: Not available.

EDC.HCl, which is commonly regarded as carbodiimide reagents, serves as a very useful tool for the formation of amide bonds (peptide bonds) in an aqueous medium. As one of the carbodiimide reagents, it is also effectively applied in anhydroxydation, polynucleotide synthesis, esterification and lactonization.In addition, EDC.HCl is also widely used as water soluble condensing reagent. [1]

In vitro: Carbodiimide-mediated coupling method was one of the most traditional approaches for peptide-bond formation. With the aim to synthesis target compounds, carbodiimide reagents were added after thoroughly mixing of a carboxylic acid and an amine. After the reaction was accomplished, the carbodiimide reagent was then changed into its corresponding urea. [2] Recently, a simple and fast detection method using spectrophotometric flow injection analysis was developed. This method was based on the reaction between EDC.HCl and pyridine in acidic aqueous solution. After reaction at 40C for a while, the absorbance was measured at 400 nm. The calibration curve demonstrated a good linearity from 0 to 10% of EDC·HCl solutions with the following regression equation: y = 3.15 × 104x (x, % concentration of EDC·HCl; y, peak area). This approach served to monitoring the concentration of EDC·HCl after its reaction in water. [1]

In vivo: So far, no in vivo data has been reported.

Clinical trial: So far, no clinical trial has been conducted.

References:
[1]Kunihiko Seno, Kazuki Matumura, Mitsuko Oshima, and Shoji Motomizu. Spectrophotometric determination of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr; 24: 505-8.
[2] Jad YE, Khattab SN, Beatriz G. Torre DL, Govender T, Kruger HG, Faham AE and Albericio F. EDC·HCl and potassium salts of oxyma and oxyma-B as superior coupling cocktails for peptide synthesis. Eur J Org Chem. 2015 May; 2015(14): 3116-20.

1. Rafiei A, Schriemer DC. "A microtubule crosslinking protocol for integrative structural modeling activities." Anal Biochem. 2019 Sep 6:113416. PMID:31499019

Physical Appearance	A solid
Storage	Desiccate at -20°C
M.Wt	191.7
Cas No.	25952-53-8
Formula	C₈H₁₇N₃·HCl
Solubility	≥19.2 mg/mL in DMSO; ≥39 mg/mL in H2O; ≥39.6 mg/mL in ETOH
Chemical Name	3-(ethyliminomethylideneamino)-N,N-dimethylpropan-1-amine;hydrochloride
SDF	Download SDF
Canonical SMILES	CCN=C=NCCCN(C)C.Cl
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Purity = 98.00%

Chemical structure

EDC.HCl [25952-53-8]

Referência A7021-10g

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