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> EGFR Protein, Human, Recombinant (His) V2

EGFR Protein, Human, Recombinant (His) V2

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Referência NB-64-128316-20ug

Tamanho : 20ug

Marca : Neo Biotech


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EGFR Protein, Human, Recombinant (His) V2

(Synonyms: PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor) Copy Product Info

Synonyms: PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor

Catalog No. TMPY-00742U Copy Product Info
EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
Contact us for more batch information

Product Information

Bioactivity
Bioactivity
Immobilized Human EGFR, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6 ng/ml determined by ELISA. (QC Test) Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator® Prime). Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay (Gator® Prime).
Description
EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP00533-1
ConstructionLeu25-Ser645
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 0.1 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsPIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
Research Background
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Chemical Properties
Molecular Weight70.19 kDa (Predicted); 80-110 kDa (Due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

TMPY-00742PIG-61PIG 61NISBD-2NISBD 2HER-1HER 1ERBB-1ERBB 1
Related Tags: EGFR Protein, Human, Recombinant (His) V2 chemical structure | EGFR Protein, Human, Recombinant (His) V2 molecular weight