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> Fluorescent DOTAP [1010076-97-7]

Fluorescent DOTAP [1010076-97-7]

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Referência HY-143702-10mg

Tamanho : 10mg

Marca : MedChemExpress


Description

Fluorescent DOTAP (NBD-DOTAP) is a cationic lipid which can be used for nucleic acid and protein delivery. Fluorescent DOTAP is labeled with a fluorophore NBD (maximum excitation/emission wavelength ∼463/536 nm). Fluorescent DOTAP can be used for gene delivery systems, drug delivery, as well as cell imaging and nanocarrier tracking. Fluorescent DOTAP is an ideal candidate for both biological and pharmaceutical formulation (e.g. co-delivery of vaccines) research[1][3].

In Vitro

Fluorescent DOTAP results in a higher hemolysis with increasing concentration of DOTAP in fusogenic liposomes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescent DOTAP Related Antibodies
In Vivo

Fluorescent DOTAP (0.5% (w/w), s.c., one single dose) increases the numbers of CD11c+ cells among total lymph node cells more than 2.5-fold compared to the untreated at 4 h in C57BL/6 female mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 female mice (6-7 weeks old) with subcutaneous tumors[3]
Dosage: 0.5% (w/w)
Administration: Subcutaneous injection (s.c.), one single dose
Result: Showed neither CD8+ or CD8- cells took up NBD after fluorescent DOTAP injection.
Demonstrated the main uptake is by DC (80%) soon after immunization.
Induced migration of activated DC to the DLN.
Resulted in DC-T cell interactions and eliciting T cell responses.
Masse moléculaire

710.34

Formule

C36H60ClN5O7
CAS No.
Appearance

Solid

Color

Orange to reddish brown

SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C21)=O)C[N+](C)(C)C)=O.[Cl-]
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvant et solubilité
In Vitro: 

DMSO : 20 mg/mL (28.16 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4078 mL 7.0389 mL 14.0778 mL
5 mM 0.2816 mL 1.4078 mL 2.8156 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2 mg/mL (2.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Pureté et documentation
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