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> GD3 Ganglioside (sodium)

GD3 Ganglioside (sodium)

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Referência HY-157343A-1mg

Tamanho : 1mg

Marca : MedChemExpress


Description

GD3 Ganglioside sodium is an Apoptosis inducer and biomarker for mouse neural stem cells. GD3 Ganglioside sodium expresses in neural stem cells and the subventricular zone of the adult mouse brain. GD3 Ganglioside sodium targets the mitochondrial permeability transition pore complex, induces pore opening, dissipates mitochondrial transmembrane potential, triggers Mitochondrial swelling, releases pro-apoptotic factors, and activates Caspase-9. GD3 Ganglioside sodium is applicable to research related to glioblastoma[1][2][3].

IC50 & Target[1]

Caspase-9

 

In Vitro

GD3 ganglioside (20 μM) sodium induces rapid swelling of isolated rat liver mitochondria by opening the mitochondrial permeability transition pore complex[2].
GD3 ganglioside (200 μM; 6 h) sodium induces caspase 9 activation in empty vector-transfected T-cell lymphoma CEM cells, and this effect is completely abrogated by forced expression of bcl-2[2].
GD3 ganglioside (200 μM; up to 18 h) sodium induces time-dependent apoptosis in empty vector-transfected T-cell lymphoma CEM cells, whereas a significant delay in apoptosis is observed in CEM cells overexpressing bcl-2[2].
GD3 ganglioside (10-40 μM) sodium induces cytochrome c release and caspase-9 activation in isolated mouse liver mitochondria[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

GD3 Ganglioside (bovine brain) sodium Related Antibodies

Western Blot Analysis[2]

Cell Line: T cell lymphoma CEM cells
Concentration: 200 μM
Incubation Time: 6 h
Result: Induced degradation of pro-caspase 9 and appearance of the 32 kDa cleavage product in empty vector-transfected CEM cells.
Did not induce caspase 9 activation in bcl-2-overexpressing CEM cells.

Apoptosis Analysis[2]

Cell Line: T cell lymphoma CEM cells
Concentration: 200 μM
Incubation Time: up to 18 h
Result: Induced apoptosis in empty vector-transfected CEM cells, with hypodiploid nuclei increasing over time.
Substantially delayed apoptosis in bcl-2-overexpressing CEM cells.
Appearance

Solid

Color

White to off-white

SMILES

[GD3 Ganglioside (bovine brain) (sodium)]
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Pureté et documentation
Références