Ghrelin Antibody

Referência ASM10553-STR-100

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Marca : Abcepta

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  • WB - Ghrelin Antibody ASM10553
    Western blot analysis of Rat Pancreas showing detection of ~12.9 kDa Ghrelin protein using Rabbit Anti-Ghrelin Polyclonal Antibody (ASM10553). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody (ASM10553) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~12.9 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q9UBU3
Other Accession NP_001128413.1
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Description Rabbit Anti-Human Ghrelin Polyclonal
Target/Specificity Detects ~13 kDa.
Other Names Appetite-regulating hormone Antibody, ghrl Antibody, Ghrelin 28 Antibody, Protein M46 Antibody, GHRL_HUMAN Antibody, GHRL Antibody, Ghrelin Antibody, Growth hormone-releasing peptide Antibody, Motilin-related peptide Antibody, Appetite regulating hormone Antibody, Growth hormone releasing peptide Antibody, Growth hormone secretagogue Antibody, Motilin related peptide Antibody, Ghrelin/obestatin prepropeptide Antibody, Obestatin Antibody, MTLRP Antibody, Ghrelin 27 Antibody, M46 protein Antibody
Immunogen Synthetic peptide from the mid-protein of Human Ghrelin
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-696 was sufficient for detection of Ghrelin in 15 µg of rat Pancreas cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Secreted