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> GluN2A/NR2A Antibody

GluN2A/NR2A Antibody

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Referência ASM10263-P594-100

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  • ICC/IF - GluN2A/NR2A Antibody ASM10263
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (ASM10263). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (ASM10263) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluN2A/NR2A Antibody (D) Composite.
  • WB - GluN2A/NR2A Antibody ASM10263
    Western Blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (ASM10263). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (ASM10263) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession Q00959
Other Accession NP_036705.3
Host Mouse
Isotype IgG2A
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GluN2A/NR2A Monoclonal IgG2A
Target/Specificity Detects ~170kDa. Does not react with NR2B.
Other Names NMDA 2A Antibody, NMDAR2A Antibody, NMDAR 2A Antibody, NMDA Receptor 2A Antibody, Glutamate Receptor Antibody, GRIN2A Antibody, Glutamate [NMDA] Receptor subunit epsilon-1 Antibody, Glutamate receptor ionotropic N methyl D aspartate 2A Antibody, HNR2A Antibody, N methyl D aspartate receptor channel Antibody, subunit epsilon 1 Antibody, N Methyl D Aspartate Receptor Subtype 2A Antibody, N methyl D aspartate receptor subunit 2A Antibody, NMDA receptor subtype 2A Antibody, NMDA Receptor Type 2A Antibody, OTTHUMP00000160135 Antibody, OTTHUMP00000174531 Antibody
Clone Names S327-95
Immunogen Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-429 was sufficient for detection o GluN2A/NR2A in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cell Junction