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> GM-CSF(BVD2-21C11), 0.2mg/mL

GM-CSF(BVD2-21C11), 0.2mg/mL

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Referência BNUB0662-100

Tamanho : 100uL

Marca : Biotium

GM-CSF(BVD2-21C11), 0.2mg/mL

GM-CSF Monoclonal Rat Antibody (BVD2-21C11)

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Product Description

Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email [email protected] to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0662, Anti-GM-CSF (BVD2-21C11) 

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC74CF®740742/767633-685775/50CF®740 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free		N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
Technology
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CF® Dyes
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Considerations for Immunofluorescence Staining
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Primary & Secondary Antibody Conjugates

Product Attributes

Antibody number
#0662
Antibody reactivity (target)
GM-CSF
Antibody type
Primary
Host species
Rat
Clonality
Monoclonal
Clone
BVD2-21C11
Isotype
IgG2a, kappa
Molecular weight
22 kDa
Synonyms
Burst Promoting Activity; Eosinophil Colony Stimulating Factor; Molgramostin; Pluripoietin Alpha; Sargramostim
Human gene symbol
CSF2
Entrez gene ID
1437
SwissProt
P04141
Unigene
1349
Immunogen
Recombinant human GM-CSF protein
Antibody target cellular localization
Secreted (extracellular)
Species reactivity
Cynomolgus monkey, Human, Rhesus monkey
Expected antibody applications
ELISA (published for clone), Flow (intracellular) (published for clone), IF (published for clone)
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunoprecipitation: 0.5-1 ug/500 ug protein lysate, Immunofluorescence: 0.5-1 ug/mL, Immunohistology (frozen) 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Neutralization Studies order Ab without azide, Optimal dilution for a specific application should be determined by user
Positive control
Lymph node and tonsil
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas
Cytokines, Immunology
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Documents, Protocols, SDS and COA

References
Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. J Immunol (2015) 194: 5085-5093. (Flow, intracellular; IF)
  2. J Exp Med (1999) 190 (6): 875-880. (ELISA)

Citations

Note: Note: Listed references are for this antibody clone sold by Biotium and other suppliers.

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