Green Cathepsin B Kit
Referência MBS225836-25
Tamanho : 25Tests
Marca : MyBiosource
Cathepsin B staining in Jurkat T cells after treatment with inhibitors.
Jurkat cells were treated with 10 ?M CA-074Me for 3 hours (blue histogram), 10?M Leupeptin for 1 hour (red histogram) or were untreated (green histogram). They were then stained for 1 hour with R110-(RR)2. After incubation with the R110-(RR)2 substrate, the samples were analyzed by flow cytometry using a ZE5 Cell Analyzer (488 nm excitation and a 525/35 emission filter). Treatment with CA-074Me or Leupeptin decreased the fluorescence signal detected compared to the untreated control (see table), which displayed a baseline level of cathepsin activity
Jurkat cells were treated with 10 ?M CA-074Me for 3 hours (blue histogram), 10?M Leupeptin for 1 hour (red histogram) or were untreated (green histogram). They were then stained for 1 hour with R110-(RR)2. After incubation with the R110-(RR)2 substrate, the samples were analyzed by flow cytometry using a ZE5 Cell Analyzer (488 nm excitation and a 525/35 emission filter). Treatment with CA-074Me or Leupeptin decreased the fluorescence signal detected compared to the untreated control (see table), which displayed a baseline level of cathepsin activity
Cathepsin B (CATB-2744) Kit
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS225836Description: Green Cathepsin B Kit enables the quantitation and monitoring of intracellular cathepsin activity over time in vitro. The Rhodamine 110 Cathepsin B substrate reagent is a non-cytotoxic and membrane permeant substrate that fluoresces green upon… More details
Product Overview
Online Datasheet in PDFProduct Name
Cathepsin B (Catb-2744), Kit
Full Product Name
Green Cathepsin B Kit
Product Gene Name
Catb-2744 kit
Product Synonym Names
Cathepsin B
Form/Format
Rhodamine 110-(RR)2
Preparation and Storage
MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL.
Store the unopened kit (and each unopened component) according to the storage instructions on each component label.
Store the Rhodamine110-(RR)2 substrate at -20 degree C.
Once reconstituted in DMSO, use the Rhodamine110-(RR)2 substrate immediately or aliquot and store at -20 degree C for 6 months, protected from light.
Avoid repeated freezing and thawing.
Store the unopened kit (and each unopened component) according to the storage instructions on each component label.
Store the Rhodamine110-(RR)2 substrate at -20 degree C.
Once reconstituted in DMSO, use the Rhodamine110-(RR)2 substrate immediately or aliquot and store at -20 degree C for 6 months, protected from light.
Avoid repeated freezing and thawing.
Product Categories/Family for Catb-2744 kit
Kits
Applications Tested/Suitable for Catb-2744 kit
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Testing Data of Catb-2744 kit
Cathepsin B staining in Jurkat T cells after treatment with inhibitors.
Jurkat cells were treated with 10 ?M CA-074Me for 3 hours (blue histogram), 10?M Leupeptin for 1 hour (red histogram) or were untreated (green histogram). They were then stained for 1 hour with R110-(RR)2. After incubation with the R110-(RR)2 substrate, the samples were analyzed by flow cytometry using a ZE5 Cell Analyzer (488 nm excitation and a 525/35 emission filter). Treatment with CA-074Me or Leupeptin decreased the fluorescence signal detected compared to the untreated control (see table), which displayed a baseline level of cathepsin activity
Jurkat cells were treated with 10 ?M CA-074Me for 3 hours (blue histogram), 10?M Leupeptin for 1 hour (red histogram) or were untreated (green histogram). They were then stained for 1 hour with R110-(RR)2. After incubation with the R110-(RR)2 substrate, the samples were analyzed by flow cytometry using a ZE5 Cell Analyzer (488 nm excitation and a 525/35 emission filter). Treatment with CA-074Me or Leupeptin decreased the fluorescence signal detected compared to the untreated control (see table), which displayed a baseline level of cathepsin activity
Other Notes
Small volumes of Catb-2744 kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Catb-2744 purchase
MBS225836 is a kit targeting Catb-2744. Also known as Green Cathepsin B Kit.
Additional Details
NCBI/Uniprot data below describe general gene information for Catb-2744. It may not necessarily be applicable to this product.
GenBank Nucleotide #
NCBI Official Full Name
cathepsin B
NCBI Official Symbol
Catb-2744
Protein Family
Cathepsin B-like cysteine proteinase
NCBI Protein Information
cathepsin B
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.