Griffonia simplicifolia GS II (Bandeiraea simplicifolia)

This lectin is a tetrameric glycoprotein composed of four subunits of nearly identical size. Each 30,000D subunit has a binding site for alpha- or beta-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively alpha- or beta-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.

Applications:
User-Optimized

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Inhibiting/Eluting Sugar:
Chitin Hydrolysate or 200mM N-acetylglucosamine

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.