Disease:

Carcinoma

Origin:

Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining.

Species:

Homo sapiens, human

Tissue:

HeLa contaminant

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Occasional polyploids. Several marker chromosomes were observed along with frequent minutes, and often 2 large chromosomes with subterminal centromeres.HeLa Marker Chromosomes: One copy of M2, two-four copies of M3 and one copy of M4 as revealed by G-banding patterns.

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37°C. A subcultivation ratio of 1:4 to 1:10 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

Complete growth culture medium 95%; DMSO, 5% (v/v) DMSO