Hepatic Co-Culture Model Cryopreserved Kit, Human/Human

Human/Human Hepatic Co-Culture Model Cryopreserved Kit

Cryopreserved adult and neonatal hepatocytes require special handling to ensure optimal viability following the freeze/thaw process. Please follow these methods closely to ensure the best performance of human hepatocytes in culture.

Kit Components:
1x vial Cryopreserved Human Hepatocytes
1x vial Cryopreserved Growth Arrested Human Liver Stromal Cells.
1x Bottle of Cryopreserved Hepatocyte Recovery Media
1x Bottle of Hepatocyte Plating Media
1x Bottle of Hepatocyte Culture Media

Required Equipment:
1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2.
2. Incubator should be controlled for humidity, temperature and CO2 concentration such as a NuAire 4950 with the following settings
a. 6% CO2
b. 95% humidity
c. 37°C
Note: It is best to fyrite incubators regularly to ensure appropriate gas concentration is maintained.

Required Supplies:
1. Fresh or cryopreserved Human hepatocytes
2. Cryopreserved growth arrested Human liver stromal cells
3. Cryopreserved Hepatocyte Recovery Media
4. Hepatocyte Plating Media
5. Hepatocyte Culture Medium
6. Collagen I coated plates
7. Liver biomatrix coated plates

Storage and Stability:
Upon receipt of the cells, immediately transfer the vials into your liquid nitrogen freezer.