HSV-1 Glycoprotein E (gE)
Referência MBS430552-5x0,05mg
Tamanho : 5x0.05mg
Marca : MyBiosource
HSV-1 Glycoprotein E (Ge) (HSV-1) Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS430552Recombinant HSV-1 glycoprotein E expressed andpurified from 293 cells. C-terminal 6xhis-tagged recombinant gE (HSV-1) (amino acid 21-419) (GenBank accession#: P04488)Introduction: Glycoprotein E (gE) of HSV is involved incell-to-cell spread and virus-induced… More details
Product Overview
Online Datasheet in PDFProduct Name
HSV-1 Glycoprotein E (gE), Recombinant Protein
Full Product Name
HSV-1 Glycoprotein E (gE)
Product Gene Name
HSV-1 recombinant protein
Host
HEK293 cells
Purity/Purification
>95% pure by 10% SDS-PAGE gel
Concentration
1mg/ml in PBS (lot specific)
Preparation and Storage
Store at -20 degree C
Stable for two weeks from the date of shipment when kept at 4 degree C. Non-hazardous. No MSDS required.
Stable for two weeks from the date of shipment when kept at 4 degree C. Non-hazardous. No MSDS required.
Applications Tested/Suitable for HSV-1 recombinant protein
Western Blot (WB) standard, antibody ELISA (EIA), Immunogen
Other Notes
Small volumes of HSV-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for HSV-1 purchase
MBS430552 is a recombinant protein targeting HSV-1. Also known as HSV-1 Glycoprotein E (gE). Host: HEK293 cells.
Additional Details
NCBI/Uniprot data below describe general gene information for HSV-1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
envelope glycoprotein E
NCBI Official Symbol
US8
UniProt Protein Name
Envelope glycoprotein E
UniProt Synonym Protein Names
gE-1
UniProt Gene Name
gE
UniProt Synonym Gene Names
gE
UniProt Entry Name
GE_HHV11
NCBI Protein Information
type 1 membrane protein; contains a signal peptide; complexed with envelope glycoprotein I to form an Fc-receptor; envelope glycoprotein E
UniProt Comments for HSV-1
Function: In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells
By similarity. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips. Ref.5 Ref.7The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses. Ref.5 Ref.7
Subunit structure: Interacts with gI; this interaction enhances the Fc receptor function of gE
By similarity. The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein. Ref.5 Ref.8 Ref.9 Ref.10
Subcellular location: Virion membrane; Single-pass type I membrane protein
By similarity. Host cell membrane; Single-pass type I membrane protein
By similarity. Host cell junction. Host Golgi apparatus membrane; Single-pass membrane protein
By similarity. Host endosome membrane; Single-pass membrane protein
By similarity. Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein. The heterodimer gE/gI then redistributes to cell junctions to promote cell-cell spread later in the infection.
Post-translational modification: Phosphorylated on serines within the acidic cluster
Potential. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane
Potential. Ref.6N-glycosylated, and sulfated. Ref.4
Sequence similarities: Belongs to the alphaherpesvirinae glycoprotein E family.
Show more By similarity. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips. Ref.5 Ref.7The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses. Ref.5 Ref.7
Subunit structure: Interacts with gI; this interaction enhances the Fc receptor function of gE
By similarity. The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein. Ref.5 Ref.8 Ref.9 Ref.10
Subcellular location: Virion membrane; Single-pass type I membrane protein
By similarity. Host cell membrane; Single-pass type I membrane protein
By similarity. Host cell junction. Host Golgi apparatus membrane; Single-pass membrane protein
By similarity. Host endosome membrane; Single-pass membrane protein
By similarity. Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein. The heterodimer gE/gI then redistributes to cell junctions to promote cell-cell spread later in the infection.
Post-translational modification: Phosphorylated on serines within the acidic cluster
Potential. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane
Potential. Ref.6N-glycosylated, and sulfated. Ref.4
Sequence similarities: Belongs to the alphaherpesvirinae glycoprotein E family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.