Quantitative, sandwich-based, glass slide antibody array to detect 10 human angiogenic factors. Suitable for all liquid sample types.
Human Angiogenesis Array Q1
Human Angiogenesis Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Angiogenesis, HER/ErbB Signaling, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| Angiopoietin-2
| EGF
| bFGF
| HB-EGF
|
HGF
| Leptin
| PDGF-BB
| PLGF
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Angiogenesis Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-ANG-1.gal)
Excel (QAH-ANG-1_Analysis_Tool.xls)
citations
Antimicrobial and Anticancer Activities of Lactiplantibacillus plantarum Probio87 Isolated from Human Breast Milk
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media from HeLa, CaSki, or C-33A cells)
- Year:
- 2025
- Journal Name:
- Nutrients
- PubMed ID:
- 40806138
Imaging of Angiogenesis in White Matter Hyperintensities
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of the American Heart Association
Endothelial extracellular vesicles enhance vascular self-assembly in engineered human cardiac tissues
- Species:
- Human
- Year:
- 2024
- PubMed ID:
- 39226913
Myocardial repair of bioengineered cardiac patches with decellularized placental scaffold and human-induced pluripotent stem cells in a rat model of myocardial infarction
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium from hiPSC-CMs on DP or matrigel)
- Year:
- 2021
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 33413626
Cord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative properties
- Species:
- Human
- Sample Type:
- Other / Unspecified (pre-cornea, cornea, post-cornea fractions)
- Year:
- 2021
- Journal Name:
- Scientific Reports
- PubMed ID:
- 34215804
In Situ Preconditioning of Human Mesenchymal Stem Cells Elicits Comprehensive Cardiac Repair Following Myocardial Infarction
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium from BM-MSC, HGF-eMSC)
- Year:
- 2021
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 33535594
Overexpression of Angiogenic Factors and Matrix Metalloproteinases in the Saliva of Oral Squamous Cell Carcinoma Patients: Potential Non-Invasive Diagnostic and Therapeutic Biomarkers
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (OSCC patients); Saliva
- Year:
- 2021
- Journal Name:
- Research Square
Synergistic anti-hepatoma effect of bufalin combined with sorafenib via mediating the tumor vascular microenvironment by targeting mTOR/VEGF signaling
- Species:
- Mouse
- Sample Type:
- Conditioned Media (HCC cells)
- Year:
- 2018
- Journal Name:
- International Journal of Oncology
Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis.
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2016
- Journal Name:
- Advances in Healthcare Materials
- PubMed ID:
- 26924785
Vascular toxicity of multi-walled carbon nanotubes targeting vascular endothelial growth factor
- Species:
- Human
- Sample Type:
- human umbilical vein endothelial cell (HUVECs)
- Year:
- 2022
- Journal Name:
- Nanotoxicology