human EchoVirus IgM, ECHO IgM ELISA Kit

Principle of the Assay||This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human echoVirus (ECHO) antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ECHO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS703446 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EchoVirus IgM, ECHO IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ECHO. The ELISA analytical biochemical technique of the MBS703446 kit is based on ECHO antibody-ECHO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ECHO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ECHO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).