ELH-GDF15-1 | Human GDF-15 ELISA Kit Clinisciences

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Human GDF-15 ELISA Kit Standard Curve

Human GDF-15 ELISA Kit

RayBio® Human GDF-15 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Sensitivity	2 pg/ml Need more sensitivity? Check out the new NexaTag^® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	2 pg/ml - 800 pg/ml
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)	3 - 30 fold
Accession Number	Q99988
Gene Id	9518
Gene Symbols	GDF15, PDF, MIC1, PLAB, PTGFB
Protein Name / Synonyms	Growth/differentiation factor 15 (GDF-15) (Macrophage inhibitory cytokine 1) (MIC-1) (NSAID-activated gene 1 protein) (NAG-1) (NSAID-regulated gene 1 protein) (NRG-1) (Placental TGF-beta) (Placental bone morphogenetic protein) (Prostate differentiation factor)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Shipping Type	Blue ice
Storage	≤-20°C

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Target Background

Growth/differentiation factor 15 (GDF-15) is a divergent member of the TGF-β superfamily of proteins (aliases: MIC-1, NAG-1, pTGFB, PDF and PLAB). Mature GDF-15 is released as a homodimer where each dimer is 25 kDa. The expression of GDF-15 is most prominent in the placenta, followed by the prostrate. Outside of the reproductive organs, it shows low constitutive expression, however, expression can be induced by stress. Cell types that produce GDF-15 include, cardiomyocytes, adipocytes, macrophages, endothelial cells and vascular smooth muscle cells. As a stress-induced cytokine, it is regulated by inflammation and stress-related proteins. The effects of GDF-15 are seen during tissue injury, metabolic conditions, pregnancy (immune tolerance) and various cancers.

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

The Human GDF15 ELISA Kit was used to quantify elevated plasma GDF15 levels in tumor-bearing mice in a cancer cachexia model. Figure adapted from Khatri et al., Cancer Letters, 2025.

The Human GDF15 ELISA Kit was used to quantify cerebrospinal fluid GDF15 levels across varying stages of HIV-associated neurocognitive impairment. Figure adapted from Boustani et al., Brain Sciences, 2025.

The Human GDF15 ELISA Kit was used to quantify serum GDF15 levels in gastric cancer patients, which were used to stratify patients for overall survival analysis. Figure adopted from Wang et al., Cancer Science, 2023.

The Human GDF15 ELISA Kit was used to quantify plasma GDF-15 levels across stages of diabetic kidney disease, demonstrating increased levels with worsening albuminuria. Figure adapted from Hussain et al., Diabetes Research and Clinical Practice, 2020.

The Human GDF15 ELISA Kit (ELH-GDF15) was used to quantify serum GDF15 levels in patients with non-small-cell lung carcinoma, confirming significantly elevated concentrations compared to healthy controls. Figure adapted from Cai et al., Cytokine, 2020.

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Additional Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human GDF-15 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified GDF-15 (GDF15), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-GDF15.pdf)

SDS (ELH-GDF15.pdf)

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

Selpercatinib mitigates cancer cachexia independent of anti-tumor activity in the HT1080 tumor model
Year:
2025
Journal Name:
Cancer Letters
PubMed ID:
39778760
Gender-specific classification of subclinical liver and renal dysfunction in older adults using machine learning and cytokine profiling
Species:
Human
Sample Type:
Serum (older adults)
Year:
2026
Journal Name:
Digital health
PubMed ID:
41659055
Associations of growth differentiation factor 15 with kidney function in frail and pre-frail older individuals: the mediating role of inflammation and oxidative stress
Species:
Human
Sample Type:
Plasma (frail and pre-frail older individuals)
Year:
2025
Journal Name:
European journal of medical research
PubMed ID:
41345886
Clinical value of SFRP1, GDF-15, and RBP in the early diagnosis and prognosis of diabetic kidney disease
Species:
Human
Sample Type:
Serum (diabetic kidney disease patients)
Year:
2025
Journal Name:
Research Square
Epicardial adipose tissue and muscle distribution affect outcomes in very old patients after transcatheter aortic valve replacement
Species:
Human
Sample Type:
Plasma (very old TAVR patients)
Year:
2024
Journal Name:
European heart journal open
PubMed ID:
39310722
Growth differentiation factor 15 induces cisplatin resistance through upregulation of xCT expression and glutathione synthesis in gastric cancer
Species:
Human
Sample Type:
Serum
Year:
2023
Journal Name:
Cancer Science
Increased Growth Differentiation Factor 15 Levels Are Associated with HIV-Associated Neurocognitive Impairment: A Pilot Study
Year:
2025
Journal Name:
Brain Sci
PubMed ID:
39851417
Conditioned Media from Head and Neck Cancer Cell Lines and Serum Samples from Head and Neck Cancer Patients Drive Catabolic Pathways in Cultured Muscle Cells
Species:
Human
Sample Type:
Plasma
Year:
2023
Journal Name:
Cancers
Hyperactive behaviour of growth differentiation factor- 15 (GDF-15) in conjunction with iron trafficking transporters and suppression of Nrf-2 gene in diabetes and metabolic syndrome
Species:
Human
Sample Type:
Plasma
Year:
2023
Journal Name:
Molecular and Cellular Biochemistry
Moderate-intensity constant or high-intensity interval training Metabolic effects on candidates to undergo bariatric surgery
Year:
2024
Journal Name:
Nutr Metab Cardiovasc Dis
PubMed ID:
38553359

Human GDF-15 ELISA Kit

Referência ELH-GDF15-1

Marca : RayBiotech

Human GDF-15 ELISA Kit