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> Human IL-1 beta ELISA Kit

Human IL-1 beta ELISA Kit

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Referência ELH-IL1b-5

Tamanho : 5PlateKit

Marca : RayBiotech


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Human IL-1 beta ELISA Kit Standard CurveHuman IL-1 beta ELISA KitHuman IL-1 beta ELISA KitELH-IL1b was used to measure IL-1β in THP-1 cell supernatants following LPS stimulation and treatment with AVR-121 or AVR-123. Figure adapted from Dayoub et al., Cells, 2024.ELH-IL1b was used to measure IL-1β in whole blood cell lysates following LPS stimulation before and after brexanolone infusion in patients with post-partum depression. Figure adapted from Balan et al., eBioMedicine, 2023.ELH-IL1b was used to measure IL-1β in HUVEC culture supernatants following exposure to silica nanoparticles. Figure adapted from Duan et al., International Journal of Nanomedicine, 2014.ELH-IL1b was used to measure IL-1β in OC3 oral squamous cell carcinoma culture media following treatment with caffeic acid and rosmarinic acid, with or without TNF-α stimulation. Figure adapted from Lee et al., Int. J. Mol. Sci., 2023.ELH-IL1b was used to measure IL-1β in hiPSC-derived cardiomyocyte culture supernatants following 6- and 48-hour exposure to bacterial flagellin. Figure adapted from Yuecel et al., Int. J. Mol. Sci., 2023.Human IL-1 beta ELISA KitHuman IL-1 beta ELISA KitHuman IL-1 beta ELISA Kit5 Plate Kit Packaging (one box)

Human IL-1 beta ELISA Kit Standard Curve

Human IL-1 beta ELISA Kit

RayBio® Human IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
0.3 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.3 pg/ml - 100 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P01584
Gene Id
3553
Gene Symbols
IL1B, IL1F2
Protein Name / Synonyms
Interleukin-1 beta (IL-1 beta) (Catabolin)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Inflammation, Antibody Drug Development, Protein Therapeutics
Shipping TypeBlue ice
Storage≤-20°C

Target Background

Interleukin-1 beta (IL-1b) plays a critical role in immune and inflammatory responses. It is produced in response to infection, inflammatory agents, and endotoxins. It also plays an important role in bone remodeling, fever, and metabolism of carbohydrates. Increased production of IL-1b is responsible for a variety of autoinflammatory syndromes.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

ELH-IL1b was used to measure IL-1β in THP-1 cell supernatants following LPS stimulation and treatment with AVR-121 or AVR-123. Figure adapted from Dayoub et al., Cells, 2024.

ELH-IL1b was used to measure IL-1β in whole blood cell lysates following LPS stimulation before and after brexanolone infusion in patients with post-partum depression. Figure adapted from Balan et al., eBioMedicine, 2023.

ELH-IL1b was used to measure IL-1β in HUVEC culture supernatants following exposure to silica nanoparticles. Figure adapted from Duan et al., International Journal of Nanomedicine, 2014.

ELH-IL1b was used to measure IL-1β in OC3 oral squamous cell carcinoma culture media following treatment with caffeic acid and rosmarinic acid, with or without TNF-α stimulation. Figure adapted from Lee et al., Int. J. Mol. Sci., 2023.

ELH-IL1b was used to measure IL-1β in hiPSC-derived cardiomyocyte culture supernatants following 6- and 48-hour exposure to bacterial flagellin. Figure adapted from Yuecel et al., Int. J. Mol. Sci., 2023.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

GCLP Validation

This assay is currently available for GCLP or Good Clinical Laboratory Practice testing service. Please contact technical support at techsupport@raybiotech.com for more details if you are interested in scheduling a GCLP testing service for this assay.

Customize This Human IL-1 beta ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified IL-1 beta (IL-1F2 / IL1B), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-IL1b.pdf)
SDS (ELH-IL1b.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • HPV16-Expressing Tumors Release Multiple IL1 Ligands to Orchestrate Systemic Immunosuppression Whose Disruption Enables Efficacy of a Therapeutic Vaccine

    Species:
    Human
    Sample Type:
    Serum (cervical cancer patients vs normal donors)
    Year:
    2025
    Journal Name:
    Cancer Discovery
    PubMed ID:
    40287949

  • Targeting polymicrobial skin infections in atopic dermatitis: role of Pichia kudriavzevii postbiotics as innovative tools for human health

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (cell culture supernatants HaCaT)
    Year:
    2026
    Journal Name:
    Frontiers in Microbiology
    PubMed ID:
    42625856

  • A 3D Alginate-Gelatin Co-Culture Model to Study Epithelial-Stromal Interactions in the Gut

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (HT-29/IMR-90 3D co-culture)
    Year:
    2026
    Journal Name:
    Gels (Basel, Switzerland)
    PubMed ID:
    41590095

  • Adjunctive effect of gaseous ozone on inflammatory biomarkers and microbial profile in grade C molar-incisor pattern periodontitis: a randomized split-mouth placebo-controlled clinical trial

    Species:
    Human
    Sample Type:
    Other Body Fluid (sample strips eluate gingival crevicular fluid)
    Year:
    2026
    Journal Name:
    BMC oral health
    PubMed ID:
    41942979

  • Late-pregnancy air pollution exposure and neonatal anthropometry with and without gestational hypertension

    Species:
    Human
    Sample Type:
    Serum (maternal serum); Whole Blood (umbilical cord blood)
    Year:
    2026
    Journal Name:
    iScience
    PubMed ID:
    42602318

  • Effects of Regular Exercise on the Biochemical, Oxidative, and Inflammatory Profiles and Quality of Life in Older Spaniards with Metabolic Syndrome

    Species:
    Human
    Sample Type:
    Plasma (older adults with metabolic syndrome)
    Year:
    2024
    Journal Name:
    Antioxidants
    PubMed ID:
    38671898

  • Non-invasive electromechanical assessment during atrial fibrillation identifies underlying atrial myopathy alterations with early prognostic value

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Nature Communications

  • Increased expression of IL-1β in adipose tissue in obesity influences the development of colon cancer by promoting inflammation

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (adipocyte-conditioned medium ACM); Whole Blood (blood circulating levels)
    Year:
    2025
    Journal Name:
    Journal of Physiology and Biochemistry
    PubMed ID:
    39305371

  • Brexanolone therapeutics in post-partum depression involves inhibition of systemic inflammatory pathways

    Species:
    Human
    Sample Type:
    blood cell
    Year:
    2023
    Journal Name:
    EBioMedicine

  • Exploring the predictive potentials of IL-1β and TNFR1 in atherogenic risk in prediabetes

    Species:
    Human
    Sample Type:
    Serum (56 prediabetic patients)
    Year:
    2025
    Journal Name:
    Scientific Reports
    PubMed ID:
    41145543

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