RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human Leptin ELISA Kit
Human Leptin ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 400 pg/ml |
| Specificity | The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 50 fold |
| Accession Number | P41159 |
| Gene Id | 3952 |
| Gene Symbols | LEP, OB, OBS |
| Protein Name / Synonyms | Leptin (Obese protein) (Obesity factor) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human Leptin qIPCR ELISA Kit
As low as $749.00
Target Background
Leptin is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. It is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Proteomic aptamer analysis reveals serum biomarkers associated with disease mechanisms and phenotypes of systemic sclerosis
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Blenderized watermelon consumption decreases body mass index, body mass index percentile, body fat and HbA1c in children with overweight or obesity
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Pediatric Obesity
How much obesity and diabetes do impair male fertility?
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Reproductive Biology and Endocrinology
Lipid profile after omega-3 supplementation in neonates with intrauterine growth retardation: a randomized controlled trial
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Pediatric Research
Familial partial lipodystrophy type 2 and obesity, two adipose tissue pathologies with different inflammatory profiles
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Diabetology & Metabolic Syndrome
Comparison of Pulmonary Function and Inflammation in Children/Adolescents with New-Onset Asthma with Different Adiposity Statuses
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Nutrients
Association between PTPN1 polymorphisms and obesity-related phenotypes in European adolescents: influence of physical activity
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Pediatric Research
Effect of fasting on short-term visual plasticity in adult humans
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- European Journal of Neuroscience
Cardiac rehabilitation may influence leptin and VEGF A crosstalk in patients after acute coronary syndrome
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Scientific reports
Periodontal status and whole salivary adipokines after scaling and root planing with and without adjunct antimicrobial photodynamic therapy in obese patients with periodontitis
- Species:
- Human
- Sample Type:
- whole salivary
- Year:
- 2022
- Journal Name:
- Photodiagnosis and Photodynamic Therapy
