- An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
- Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
- Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
- Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
- HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
Human Lipopolysaccharides,LPS ELISA Kit
Referência CSB-E09945h-96T
Tamanho : 96T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Lipopolysaccharides,LPS
Abbreviation
LPS
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
Recovery
| The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-97 | ||||
| EDTA plasma (n=4) | 88 | 82-96 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human LPS ELISA Kit was designed for the quantitative measurement of Human LPS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .
Citations
- X Yan, X Zhang, L Wang, W Song, T Qi,Frontiers in Cellular and Infection Microbiology,2026
- H Liang, X Ding, S Liu, S Tong, X Wang, Z Zhang,Gut Microbes,2026
- L Xie, R Zhang, H Zou, J Hu, J Deng, J Ding, L Liu,Science Advances,2026
- A Simón Rueda, C Sánchez-Menéndez,Frontiers in Immunology,2025
- S Yang, S Feng, H Wu, C Zhong, S Zhan,Clinical Interventions in Aging,2025
- L Loffredo, E Maggio, S Bartimoccia, A Magna,Antioxidants,2025
- A Schieren, H Huber, C Alvarez-Garavito, A Mantri,medRxiv,2025
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