RayBio® Human MMP-9 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human MMP-9 ELISA Kit Standard Curve
Human MMP-9 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 10 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 10 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 300 - 3,000 fold |
| Accession Number | P14780 |
| Gene Id | 4318 |
| Gene Symbols | MMP9, CLG4B |
| Protein Name / Synonyms | Matrix metalloproteinase-9 (MMP-9) (EC 3.4.24.35) (92 kDa gelatinase) (92 kDa type IV collagenase) (Gelatinase B) (GELB) [Cleaved into: 67 kDa matrix metalloproteinase-9 82 kDa matrix metalloproteinase-9] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. In humans the MMP9 gene encodes for a signal peptide, a propeptide, a catalytic domain with inserted three repeats of fibronectin type II domain followed by a C-terminal hemopexin-like domain.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human MMP-9 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MMP-9 (MMP9), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Immunomodulatory and Anti-Inflammatory Effects of Ketotifen Versus Vitamin E in Patients with Non-Alcoholic Fatty Liver Disease: A Randomized Pilot Study
- Year:
- 2026
- Journal Name:
- Drug Design Development and Therapy
- PubMed ID:
- 42039690
Repurposing doxycycline as an adjunct to sitagliptin in type 2 diabetes mellitus: a randomized controlled study on glycemic, inflammatory, and cardiometabolic outcomes
- Year:
- 2026
- Journal Name:
- Naunyn Schmiedebergs Arch Pharmacol
- PubMed ID:
- 42271067
Decreased ratios of matrix metalloproteinases to tissue-type inhibitors in cerebrospinal fluid in sporadic and hereditary cerebral amyloid angiopathy
- Species:
- Human
- Sample Type:
- cerebrospinal fluid (CSF)
- Year:
- 2023
- Journal Name:
- Alzheimers Research & Therapy
Anti-Metastatic Potential of Hydroalcoholic Extract of Pistacia Khinjuk Leaves and Its Mechanism: An in Vitro Study
- Species:
- Human
- Sample Type:
- Other / Unspecified (secretions of cells treated with the extract)
- Year:
- 2025
- Journal Name:
- Journal of Advances in Medical and Biomedical Research
LIPID PEROXIDATION, MMP-9 ACTIVITY, AND BDNF LEVEL IN PATIENTS WITH PARANOID SCHIZOPHRENIA DEPENDING ON THE DURATION OF THE DISEASE
- Species:
- Human
- Sample Type:
- Plasma (blood plasma)
- Year:
- 2025
Role of MMP-2, MMP-9, TIMP-1, and TIMP-2 in children with ventricular septal defect
- Year:
- 2025
- Journal Name:
- Biomol Biomed
- PubMed ID:
- 39466144
The Anti-Metastatic Potential of Aronia Leaf Extracts on Colon Cancer Cells
- Species:
- Human
- Sample Type:
- Other / Unspecified (HT-29, SW-480 cells)
- Year:
- 2024
- Journal Name:
- Nutrients
- PubMed ID:
- 39683504
Paroxysmal slow wave events are associated with cognitive impairment in patients with obstructive sleep apnea
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Alzheimers Research & Therapy
Coordination behavior of cocaine toward d-block metal ions: Synthesis, spectral analysis, density functional theory (DFT) studies, and chemotherapeutic activity
- Species:
- Human
- Sample Type:
- MCF-7 cell
- Year:
- 2023
- Journal Name:
- Journal of Molecular Structure
DHA Induces Cell Death through the Production of ROS and the Upregulation of CHOP in Fibroblast-like Synovial Cells from Human Rheumatoid Arthritis Patients
- Species:
- Human
- Sample Type:
- RA patients (RA-FLS) MH7A cell
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
