RayBio® G-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Human RTK Phosphorylation Array G1
Human RTK Phosphorylation Array G1
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 71 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Post-Translational Modifications, Phosphorylation, PI3K-AKT Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| ABL1
| ACK
| ALK-1
| Axl
| Blk
|
| BMX
| Btk
| Csk
| Dtk
| EGFR
|
| EphA1
| EphA2
| EphA3
| EphA4
| EphA5
|
| EphA6
| EphA7
| EphA8
| EphB1
| EphB2
|
| EphB3
| EphB4
| EphB6
| ErbB2
| ErbB3
|
| ErbB4
| FAK
| FER
| FGFR1
| FGFR2
|
| FGFR2 alpha isoform
| Fgr
| FRK
| Fyn
| Hck
|
| HGFR
| IGF-1 R
| Insulin R (CD220)
| Itk
| JAK1
|
| JAK2
| JAK3
| LCK
| LTK
| Lyn
|
| MATK
| M-CSF R
| MUSK
| NGFR (TNFRSF16)
| PDGFRA
|
| PDGFRB
| PYK2
| RET
| ROR1
| ROR2
|
| ROS
| RYK
| SCF R (CD117/c-kit)
| SRMS
| SYK
|
| Tec
| Tie-1
| Tie-2
| TNK1
| TRKB
|
| TXK
| Tyk2
| TYRO10 (DDR2/TKT)
| VEGFR2
| VEGFR3
|
| ZAP70
| ||||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human RTK Phosphorylation Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail II
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Development of a novel Bruton’s tyrosine kinase inhibitor that exerts anti-cancer activities potentiates response of chemotherapeutic agents in multiple myeloma stem cell-like cells
- Species:
- Human
- Sample Type:
- Human MM cell
- Year:
- 2022
- Journal Name:
- Frontiers in pharmacology
Paracrine regulation of glioma cells invasion by astrocytes is mediated by glial-derived neurotrophic factor
- Species:
- Human
- Sample Type:
- Cell Lysate (Human Glioma cell lysates)
- Year:
- 2015
- Journal Name:
- International Journal of Cancer
- PubMed ID:
- 25487790
Identification of the receptor tyrosine kinases (RTKs)-oriented functional targets of miR-206 by an antibody-based protein array
- Species:
- Human
- Sample Type:
- Cell Lysate (A549 cells with or without various MiR mimics, lung adenocarcinoma)
- Year:
- 2015
- Journal Name:
- Febs Letters
- PubMed ID:
- 26143374
Control of the MYC-eIF4E axis plus mTOR inhibitor treatment in small cell lung cancer
- Species:
- Human
- Sample Type:
- Cell Lysate (Small Cell Lung cancer cell lines (7 in total), treated with everolimus)
- Year:
- 2015
- Journal Name:
- Bmc Cancer
- PubMed ID:
- 25884680
Macrophages increase the resistance of pancreatic adenocarcinoma cells to gemcitabine by upregulating cytidine deaminase.
- Species:
- Human
- Sample Type:
- Cell Lysate (PANC-1 pancreatic cancer cell line)
- Year:
- 2013
- Journal Name:
- Oncoimmunology
- PubMed ID:
- 24498570
Using protein microarrays to study phosphorylation-mediated signal transduction
- Species:
- Human
- Sample Type:
- N/A (Review reference, no sample type, just a reference to our arrays)
- Year:
- 2012
- Journal Name:
- Seminars in Cell and Developmental Biology
- PubMed ID:
- 22706299
MP470, a novel receptor tyrosine kinase inhibitor, in combination with Erlotinib inhibits the HER family/PI3K/Akt pathway and tumor growth in prostate cancer
- Species:
- Human
- Sample Type:
- Cell Lysate (Prostate cancer cell lysate)
- Year:
- 2009
- Journal Name:
- Bmc Cancer
MP470, a novel receptor tyrosine, in combination with Erlotinib inhibits the HER family/PI3K/Akt pathway and tumor growth in prostate cancer
- Species:
- Human
- Sample Type:
- Cell Lysate (LNCaP cultured cell lysate)
- Year:
- 2009