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> In vivo Collagen Hybridizing Peptide, sulfo-Cy7.5 Conjugate

In vivo Collagen Hybridizing Peptide, sulfo-Cy7.5 Conjugate

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Referência INVIVOTGT7.5

Tamanho : 3Doses (8nmole)

Marca : 3Helix


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Description
Description
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Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

The in vivo CHP incorporates our newest sequence which allows them to be directly injected into animals without having to pre-activate them with a heating step. [4] Therefore, the in vivo CHPs will always be in their active form and will not self-hybridize once injected. [4] The sCy7.5-CHP is a powerful in vivo tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5]

The sCy7.5-CHPs are labeled with sulfonated-cyanine 7.5 dye for near-infrared fluorescence (NIRF) detection. This wavelength offers better tissue penetration while minimizing tissue autofluorescence.

Specification

Synonyms sCy7.5-CHP, collagen mimetic peptide (CMP)
Molecular weight 3703.1 g/mol
Purity 85% by HPLC
Conjugate Single sulfonated-cyanine 7.5 dye per peptide
Excitation 778 nm
Emission 797 nm
Content Purified lyophilized powder, green color
Storage -20 °C as powder (4 °C for a few days once reconstituted)

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).[3,6]

Applications: in vivo imaging, small animal imaging

Features

  • New sequence designed for in vivo use- does not need any pre-activation step unlike CHPs for histology
  • Near-infrared imaging probe minimizes the chance of autofluorescence in the gut or certain tissues
  • Can be used for systemic or in situ injection
  • Signal lasts at target sites for days, allowing for imaging at multiple time points with a single dose
  • High affinity and unparalleled specificity to collagen turnover with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Stable as powder form, stored at -20 °C

 

Additional Information

  • sCy7.5-CHP dose can be adjusted based on animal model or application. Current dosing is based on a mouse model. Please check the protocol for details:
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Citations (7)
Citations (7)
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  • Huang, K., Ren, R., Fei, Y., Zhou, D., Li, X., Liu, Z., Jiang, T., Fei, Y.X., Hou, Y., Zhou, D.N., Wang, W., Zhang, Y., Li, S., Shen, D., & Lin, Y. (2024). Targeting Molecular Collagen Defects from the Initiation of Knee Osteoarthritis. medRxiv, 2024.06.13.24308739. doi: https://doi.org/10.1101/2024.06.13.24308739.
  • Westenskow, P. D., Vettore, L., Wang, H., Schilter, H., Peluso, I., Stumpp, M. T., & Zanoni, M. (2024). In vivo monitoring of active subretinal fibrosis in mice using collagen hybridizing peptides. Lab Animal (NY), 53(8), 196–204. doi: https://doi.org/10.1038/s41684-024-01408-0.
  • Li, Y., Su, W., Li, J., Qiu, B., Li, S., Du, Y., Liu, P., & Wang, Q. (2021). Targeted Delivery of MicroRNA-29b to Activated Macrophages for Anti-Fibrosis Therapy in Hepatic Fibrosis. ACS Nano, 15(12), 19138–19149. doi: https://doi.org/10.1021/acsnano.1c07112.
  • Sun, X., Wu, M., Tan, X., Liu, S., Xu, K., Wang, Z., Lin, J., & Liu, J. (2023). Injectable self-healing hydrogel encapsulating black phosphorus nanosheets for synergistic chemo-photothermal therapy of breast cancer. Journal of Controlled Release, 353, 278–288. doi: https://doi.org/10.1016/j.jconrel.2022.10.017.
  • Sharma, A., Kumar, A., AlGhamdi, K. M., Al-Ghamdi, A. A., Kumar, M., Kumar, S., Kumar, A., & El-Shazly, M. (2023). Crosstalk between NRF2 and NF-κB in response to sub-lethal heat stress in broiler chickens (Gallus gallus). Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1866(2), 194928. doi: https://doi.org/10.1016/j.bbagrm.2023.194928.
  • Ganji, E. (2021). Growth and mechanobiology of the Achilles enthesis in mice (Doctoral dissertation, University of Delaware). Link to paper (ProQuest)
  • Li, Y., Liu, R., Wang, L., Wu, P., Lin, C., Zhao, M., Liu, M., Yu, W., Li, C., & Zhang, X. (2017). Peptide Hivenyl-Decorated Liposomes for Enhanced Delivery of Paclitaxel to Hepatocellular Carcinoma. Molecular Pharmaceutics, 14(6), 1906–1915. doi: https://doi.org/10.1021/acs.molpharmaceut.7b00009.
In Vivo | Collagen Hybridizing Peptides, sulfo-Cy7.5 Conjugates (sCy7.5-CHP)

In Vivo | Collagen Hybridizing Peptides, sulfo-Cy7.5 Conjugates (sCy7.5-CHP)

In Vivo | Collagen Hybridizing Peptides, sulfo-Cy7.5 Conjugates (sCy7.5-CHP)
In Vivo Collagen Hybridizing Peptide Staining in Subretinal Fibrosis Mouse Model
Collagen Hybridizing Peptide Multiple Myeloma In Vivo Staining

In Vivo | Collagen Hybridizing Peptides, sulfo-Cy7.5 Conjugates (sCy7.5-CHP)