Biological Activity

Jc-1 (Mitochondrial membrane potential probe) is a fluorescent lipophilic carbonyl cyanine dye used to measure mitochondrial membrane potential. In normal mitochondria, JC-1 aggregates in the mitochondrial matrix to form a polymer, which emits strong red fluorescence (Ex=585 nm, Em=590 nm); When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria and produce green fluorescence (ex=510 nm, em= 527 nm).

The maximum excitation/emission wavelength is 515nm/529nm (590nm), and green fluorescence is emitted.

Used to observe mitochondria, stored at room temperature, away from light.

Jc-1 should be redissolved in high-quality freshly opened DMSO (1 mg/mL). After redissolution, it should be stored at -20°C; Avoid freeze-thaw.

Product Citations

• 

  J Colloid Interface Sci. 2025 Oct 17; .

  Trienzyme-like activities nanoreactor boosts tumor ferroptosis and mild photothermal therapy via gas/starvation synergy
  JC-1 purchased from AbMole

• 

  Int Immunopharmacol. 2023 Sep 21;124(Pt B):110963.

  BNTA attenuates temporomandibular joint osteoarthritis progression by directly targeting ALDH3A1: An in vivo and in vitro study
  JC-1 purchased from AbMole

• 

  Life Sci. 2021 Jan 15;265:118853.

  Circadian clock gene BMAL1 reduces urinary calcium oxalate stones formation by regulating NRF2/HO-1 pathway
  JC-1 purchased from AbMole

Chemical Information

Molecular Weight	652.23
Formula	C25H27Cl4IN4
CAS Number	3520-43-2
Solubility (25°C)	DMSO ≥ 10 mg/mL
Storage	-20°C, protect from light, dry, sealed

References

[1] A Perelman, et al. Cell Death Dis. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry

[2] Vera C Keil, et al. Pflugers Arch. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria

[3] Jung-Ho Lee, et al. Biochem J. Real-time analysis of amyloid fibril formation of alpha-synuclein using a fibrillation-state-specific fluorescent probe of JC-1

[4] S Salvioli, et al. FEBS Lett. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis