Rabbit polyclonal to KDEL (PerCP). The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER..
Species/Host
Rabbit
Clonality
Polyclonal
Tested applications
ICC, IHC, WB
Reactivity
Human, Mouse, Rat
Immunogen
KDEL containing peptide immunogen
Concentration
1 mg/ml
Dilution range
WB (1:1000), ICC/IF (1:100)
Conjugation
PerCP
Target
KDEL
Storage
Conjugated antibodies should be stored at 4°C
Buffer/Preservatives
PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Alternative names
Lys Asp Glu Leu antibody
Note
For research use only
Application notes
A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.