Lipase Activity Colorimetric Assay Kit

Referência K2227-100

Tamanho : 100assays

Marca : APExBIO Technology


Lipase Activity Colorimetric Assay Kit

Catalog No.
K2227
Detects lipase activity, sensitive, high throughput screening (HTS).

Background

Lipase is an enzyme that catalyzes fats (lipids) hydrolysis and is a subclass of the esterases. Lipase plays important roles in the digestion, transport and processing of dietary lipids (e.g. oils, triglycerides, fats) in most living organisms. In human digestive system, pancreatic lipase is the key enzyme that converts triglycerides to monoglycerides and free fatty acids. In the damaged pancreas, lipase levels can rise 5 to 10-fold within 24 to 48 hours.

 The Lipase Activity Colorimetric Assay Kit provides a sensitive, easy and fast way for detection of lipase activity in various samples based on colorimetric method. In the assay, lipase in the sample hydrolyzes a triglyceride substrate to produce glycerol, which is quantified enzymatically through monitoring a linked change in the OxiRed probe absorbance (λ = 570 nm). The kit is suited for high throughput screening assay of lipase activity and can detect lipase activity as low as 10 mU/well.

Features & Properties

FeaturesFast and convenient; Simple procedure; Takes approx. 60-90 minutes; Sensitive assays for measuring lipase activity in various biological samples.
ShippingBlue Ice
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

View current batch:
 

Storage

Store at -20°C.

Overview

Kit componentsLipase Assay Buffer, OxiRed Probe (in DMSO), Enzyme Mix (lyophilized), Lipase Substrate, Glycerol Standard (100 mM), Lipase Positive Control

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Referência
Descrição
Cond.
Price Bef. VAT
STA-394
 96assays