Listeria monocytogenes Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommended Dilution	Immunohistochemistry on Frozen Sections: 1/20-1/200. Western blot: 1/20-1/200. ELISA: > 1/1000.
Reactivities	Listeria monocytogenes
Host	Rabbit
Clonality	Polyclonal
Immunogen	Listeria monocytogenes; ATCC strain #43251
Specificity	Recognizes whole cells. This antiserum has not been absorbed and may react with related microorganisms. Crossreacts with Group A Streptococcus, Group B Streptococcus, S. pneumoniae, Staph aureus, clostridium perfingens and Bacillus subtilis.
Formulation	PBS with 0.002% Thimerosal as preservative and 10 mg/ml BSA as stabilizer. Label: HRP State: Liquid purified Ig fraction. Label: Highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed Molar radio: HRP:IgG substitution is 2-3.
Concentration	lot specific
Conjugation	HRP
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Background	The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
Reference Data