Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit
Referência MBS9719385-5x96
Tamanho : 5x96Tests
Marca : MyBiosource
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity (PEPCK) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9719385PROMOTIONAL PRICING: 5% OFF web . Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed
Principle of the Assay: PEPCK (EC 4.1.1.32) is widely found in animals, plants, microorganisms and cells and catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, a key enzyme in the regulation of gluconeogenic pathways. CheKine™ Micro… More details
Product Overview
Online Datasheet in PDFProduct Name
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity, Assay Kit
Full Product Name
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit
Product Gene Name
PEPCK assay kit
Samples
Animal Tissue, Plant Tissue, Cells, Bacteria, Serum, Plasma
Preparation and Storage
Storage at –20 degree C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Usage Note
96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
Do not mix or substitute reagents or materials from other kit lots or vendors.
The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Do not mix or substitute reagents or materials from other kit lots or vendors.
The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Product Categories/Family for PEPCK assay kit
Cytology; Cell Metabolism; Biochemical Assay Kit
Application Notes for PEPCK assay kit
Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit provides a simple, convenient and rapid method for PEPCK activity detection, which is suitable for testing animal and plant tissues, cells, bacteria, serum (plasma) and other samples. The principle is that PEPCK catalyzes the oxidation of oxaloacetate to produce phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to produce NAD+ in turn, The rate of NADH decline was measured at 340 nm, which reflects PEPCK activity.
Other Notes
Small volumes of PEPCK assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PEPCK purchase
MBS9719385 is an assay kit targeting PEPCK. Also known as Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit.
Additional Details
NCBI/Uniprot data below describe general gene information for PEPCK. It may not necessarily be applicable to this product.
Protein Family
Phosphoenolpyruvate carboxykinase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.