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> Monkeypox Virus (MPXV) A29L ELISA Kit

Monkeypox Virus (MPXV) A29L ELISA Kit

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Referência ELV-MPXVA29-2

Tamanho : 2PlateKit

Marca : RayBiotech


Monkeypox Virus (MPXV) A29L ELISA KitMonkeypox Virus (MPXV) A29L ELISA KitMonkeypox Virus (MPXV) A29L ELISA Kit5 Plate Kit Packaging (one box)

Monkeypox Virus (MPXV) A29L ELISA Kit

Monkeypox Virus (MPXV) A29L ELISA Kit

The RayBiotech Monkeypox Virus (MPXV) A29L ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of MPXV protein A29L in serum, plasma, and cell culture supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesMonkeypox virus (MPXV)
Sensitivity
3.6 pg/ml
Detection Range
3.6 pg/ml - 20 ng/ml
Specificity
This ELISA antibody pair detects MPXV A29L.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
3 fold
Accession Number
Q90188
Gene Symbols
A29L, MPXV-M5312_HM12_Rivers-138
Protein Name / Synonyms
14 kDa protein (14K membrane protein) (14kDa fusion protein) (MPXVgp139) (A29L)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaInfectious Disease, Mpox
Shipping TypeBlue ice
Storage-20°C

Introduction

This assay employs an antibody specific for MPXV A29L coated on a 96-well plate. Standards and samples are pipetted into the wells and MPXV A29L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-MPXV A29L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPXV A29L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Monkeypox Virus (MPXV) A29L ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified A29, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

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