Mouse 43 kDa receptor-associated protein of the synapse (RAPSN) ELISA Kit

This assay employs a two-site sandwich ELISA to quantitate RAPSN in samples. An antibody specific for RAPSN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAPSN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAPSN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAPSN bound in the initial step. The color development is stopped and the intensity of the color is measured.