RayBio® Mouse IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
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Mouse IL-1 beta ELISA Kit
Mouse IL-1 beta ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 5 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P10749 |
| Gene Id | 16176 |
| Gene Symbols | IL1B |
| Protein Name / Synonyms | Interleukin-1 beta (IL-1 beta) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Target Background
Interleukin-1β (IL1b) is a pro-inflammatory cytokine important for immune responses to infection and injury. It is produced as a 31 kDa precursor, termed pro-IL-1b which is cleaved by caspase-1-dependent processing for secretion. IL1b is expressed in a wide variety of tissues and cells, especially in macrophages in the thymus, spleen, lymph nodes, Peyer's patches and bone marrow. Disruption of IL1b signaling can lead to inflammatory disease and malignancies.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse IL-1 beta ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-1 beta (IL-1F2 / IL1B), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Apelin-13 prevents GATA4-mediated neuronal senescence by restoring autophagy through mTORC1 inhibition
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (hippocampus)
- Year:
- 2026
- Journal Name:
- Cell Death & Disease
Acarbose modulates microglial Pkm2 acetylation to reshape immunometabolism and preserve retinal neurons after ischemia-reperfusion
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (retinas)
- Year:
- 2026
- Journal Name:
- Journal of Neuroinflammation
- PubMed ID:
- 42069589
TRIM30a coordinates neutrophil-macrophage crosstalk to resolve inflammation and drive osseointegration via suppressing NETosis/cGAS-STING axis
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (supernatant of neutrophils or macrophages)
- Year:
- 2026
- Journal Name:
- Materials Today Bio
- PubMed ID:
- 41560844
Icariin alleviates corneal neovascularization by regulating inflammation through inhibition of macrophage polarization and the NF-κB pathway
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (corneal tissue homogenate supernatant, in vitro samples)
- Year:
- 2026
- Journal Name:
- Journal of Translational Medicine
- PubMed ID:
- 41652511
A naturally derived lipopeptide lipid nanoparticle platform enabling multiple nucleic acids delivery
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2025
- Journal Name:
- Bioactive Materials
- PubMed ID:
- 41268339
Cataract Aggravates Alzheimer-Like Pathologies and Cognitive Deficits in an APP/PS1 Mouse Model
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (brain homogenates)
- Year:
- 2026
- Journal Name:
- Neuroscience Bulletin
- PubMed ID:
- 40580389
Mitochondrial reactive oxygen species promote cancer metastasis and tumor microenvironment immunosuppression through gasdermin D
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (metastatic lung tissue lysates)
- Year:
- 2025
- Journal Name:
- Cell Death Discovery
- PubMed ID:
- 40324993
Anti-Inflammatory Effects of Marine-Derived Resorcylic Acid Lactone Derivatives in Ulcerative Colitis via the MAPK/ERK Pathway
- Species:
- Mouse
- Sample Type:
- Serum (colitis mice)
- Year:
- 2026
- Journal Name:
- Marine Drugs
- PubMed ID:
- 41745472
Aging and MPTP Sensitivity Depend on Molecular and Ultrastructural Signatures of Astroglia and Microglia in Mice Substantia Nigra
- Species:
- Mouse
- Sample Type:
- Tissue Lysate / Homogenate (protein lysates from ventral midbrains)
- Year:
- 2025
- Journal Name:
- Cellular and Molecular Neurobiology
- PubMed ID:
- 39833644
The absence of thioredoxin-interacting protein in alveolar cells exacerbates asthma during obesity
- Species:
- Mouse
- Sample Type:
- Serum (obese asthma model mice)
- Year:
- 2024
- Journal Name:
- Redox Biology
- PubMed ID:
- 38781728
