Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Mouse KC ELISA Kit
Mouse KC ELISA Kit
RayBio® Mouse KC (CXCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 150 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 fold |
| Accession Number | P12850 |
| Gene Id | 14825 |
| Gene Symbols | CXCL1, GRO, GRO1, MGSA, SCYB1 |
| Protein Name / Synonyms | Growth-regulated alpha protein (C-X-C motif chemokine 1) (Platelet-derived growth factor-inducible protein KC) (Secretory protein N51) [Cleaved into: KC(5-72) (Hematopoietic synergistic factor) (HSF) (KC-T)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Mouse KC qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse KC ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified CXCL1 (GRO alpha / KC / CINC-1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
A mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (footpad lysate supernatants)
- Year:
- 2024
- Journal Name:
- EMBO Journal
- PubMed ID:
- 38378891
Identification of host lncRNAs that impact Venezuelan equine encephalitis virus TC-83 replication
- Year:
- 2026
- Journal Name:
- Journal of Virology
- PubMed ID:
- 42023950
Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation
- Species:
- Mouse
- Sample Type:
- Serum (LPS-challenged, fructose-fed mice)
- Year:
- 2021
- Journal Name:
- Nature Communications
- PubMed ID:
- 33619282
Oxidative Stress Product, 4-Hydroxy-2-Nonenal, Induces the Release of Tissue Factor-Positive Microvesicles From Perivascular Cells Into Circulation
- Species:
- Mouse
- Sample Type:
- Plasma (saphenous vein bleeding)
- Year:
- 2020
- Journal Name:
- Arteriosclerosis, Thrombosis, and Vascular Biology
Interleukin-8 as a therapeutic target for chronic low back pain: Upregulation in human cerebrospinal fluid and pre-clinical validation with chronic reparixin in the SPARC-null mouse model
- Species:
- Mouse
- Sample Type:
- Serum (protein extracts from mouse discs)
- Year:
- 2019
- Journal Name:
- eBioMedicine
- PubMed ID:
- 31047862
Krüppel-Like Factor 15 Modulates CXCL1/CXCR2 Signaling-Mediated Inflammatory Response Contributing to Angiotensin II-Induced Cardiac Remodeling
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (cell supernatant of cardiac fibroblasts, H9c2 cells)
- Year:
- 2021
- Journal Name:
- Frontiers in Cell and Developmental Biology
- PubMed ID:
- 33869197
Unique Pro-Inflammatory Response of Macrophages during Apoptotic Cancer Cell Clearance
- Species:
- Mouse
- Sample Type:
- Conditioned media (cell culture media, bone marrow derived macrophages)
- Year:
- 2020
- Journal Name:
- Cells
MicroRNA let-7 Modulates the Immune Response to Mycobacterium tuberculosis Infection via Control of A20, an Inhibitor of the NF-kB Pathway
- Species:
- Mouse
- Sample Type:
- Conditioned Media (A20-/- macrophages)
- Year:
- 2015
- Journal Name:
- Cell Host and Microbe
- PubMed ID:
- 25683052
Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines
- Species:
- Mouse
- Sample Type:
- Bronchoalveolar Lavage (BALF supernatant)
- Year:
- 2020
- Journal Name:
- Aging
- PubMed ID:
- 32756014
Genetic variation in surfactant protein-A2 alters responses to ozone
- Species:
- Mouse
- Sample Type:
- Bronchoalveolar Lavage (ozone-exposed mice)
- Year:
- 2021
- Journal Name:
- PLoS ONE
- PubMed ID:
- 33617569