Mouse Proteome Profiling Array L308 (Membrane)

Referência AAM-BLM-1-4

Tamanho : 4SampleKit

Marca : RayBiotech


Mouse Proteome Profiling Array L308 (Membrane)

Label-based multiplex assay on nitrocellulose membranes for simultaneous detection of 308 mouse proteins. Ideal for exploratory biomarker screening in serum, plasma, or cell culture supernatants.
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Product Description

Specifications

Size2 Sample Kit, 4 Sample Kit
SpeciesMouse
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected308
Solid Support
Membrane
Design PrincipleLabel-based
Method Of DetectionChemiluminescence
Shipping TypeBlue ice
StorageMixed storage. See datasheet.

Product Features

  • High density arrays
  • Unbiased detection through direct biotin labeling of samples
  • Low sample consumption
  • High detection sensitivity
  • Same day result
  • Affordable and simple to use

Target Names

6Ckine, Activin A, Activin C, Activin RIB / ALK-4, Adiponectin / Acrp30, AgRP, ALCAM, Angiopoietin-like 2, Angiopoietin-like 3, AREG (Amphiregulin), Artemin, Axl, bFGF, B7-1/CD80, BAFF R / TNFRSF13C, BCMA / TNFRSF17, beta-Catenin, BLC, BTC (Betacellulin), Cardiotrophin-1, CCL1 / I-309 / TCA-3, CCL28, CCL4 / MIP-1 beta, CCL7 / MCP-3 / MARC, CCL8 / MCP-2, CCR10, CCR3, CCR4, CCR6, CCR7, CCR9, CD11b, CD14, CRP, CD27 / TNFRSF7, CD27 Ligand / TNFSF7, CD30, CD30 L, CD40, CD40 Ligand / TNFSF5, Cerberus 1, Chordin-Like 2, Coagulation Factor III / Tissue Factor, Common gamma Chain / IL-2 R gamma, CRG-2, Cripto, Crossveinless-2, Cryptic, Csk, CTACK , CTLA-4 / CD152, CXCL14 / BRAK, CXCL16, CXCR2 / IL-8 RB, CXCR3, CXCR4, CXCR6, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, DPPIV / CD26, DR3 / TNFRSF25, Dtk, EDAR, EGF R, EG-VEGF / PK1, Endocan, Endoglin / CD105, Endostatin, Eotaxin, Eotaxin-2, Epigen, Epiregulin, Erythropoietin (EPO), E-Selectin, FADD, FAM3B, Fas / TNFRSF6, Fas Ligand, FCrRIIB / CD32b, FGF R3, FGF R4, FGF R5 beta, FGF-21, Fit-3 Ligand, FLRG (Follistatin), Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-6, Frizzled-7, Galectin-3, G-CSF, GDF-1, GDF-3, GDF-5, GDF-8, GDF-9, GFR alpha-2 / GDNF R alpha-2, GFR alpha-3 / GDNF R alpha-3, GFR alpha-4 / GDNF R alpha-4, GITR, GITR Ligand / TNFSF18, Glut2, GM-CSF, Granzyme B, Granzyme D, Granzyme G, Gremlin, Growth Hormone R, HGF R, HGF, HVEM / TNFRSF14, ICAM-1, ICAM-2 / CD102, ICAM-5, ICK, IFN-alpha / beta R1, IFN-alpha / beta R2, IFN-beta, IFN-gamma , IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGFBP-rp1 / IGFBP-7, IGF-I, IGF-II, IL-1 alpha, IL-1 beta, IL-1 R4 / ST2, IL-1 R6 / IL-1 R rp2, IL-1 R9, IL-1 RI, IL-1 RII, IL-2, IL-2 R alpha, IL-2 R beta, IL-3, IL-3 R alpha, IL-3 R beta, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-9, IL-9 R, IL-10, IL-10 R alpha, IL-11, IL-12 p40/p70, IL-12 p70, IL-12 R beta 1, IL-13, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 R alpha/IL-1 R5, IL-20, IL-20 R alpha, IL-21, IL-21 R, IL-22, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-28 / IFN-lambda, IL-31, IL-31 RA, Insulin, Integrin beta 2 / CD18, I-TAC, KC, Kremen-1, Kremen-2, Lefty-1, Leptin R, LEPTIN(OB), LIF, LIGHT / TNFSF14, LIX, LRP-6, L-Selectin, Lungkine, Lymphotactin, Lymphotoxin beta R / TNFRSF3, MAdCAM-1, MCP-1, MCP-5, M-CSF, MDC, MFG-E8, MFRP, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta , MMP-2, MMP-3, MMP-9, MMP-12, MMP-14 / LEM-2, MMP-24 / MT5-MMP, Neuregulin-3 / NRG3, Neurturin, NGF R / TNFRSF16, NOV / CCN3, Osteoactivin / GPNMB, Osteopontin, Osteoprotegerin, OX40 Ligand / TNFSF4, PDGF C, PDGF R alpha, PDGF R beta, Pentraxin3 / TSG-14, PF-4, PlGF-2, Progranulin, Prolactin, P-Selectin, RAGE, RANTES, RELM beta, Resistin, S100A10, SCF, SCF R / c-kit, SDF-1, Serum Amyloid A1, Shh-N, SIGIRR, SLPI, Soggy-1, SPARC, Spinesin Ectodomain, TACI / TNFRSF13B, TARC, TCA-3, TCCR / WSX-1, TECK, TFPI, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta RI / ALK-5, TGF-beta RII, Thrombospondin, Thymus Chemokine-1, Tie-2, TIMP-1 , TIMP-2, TIMP-4, TL1A / TNFSF15, TLR1, TLR2, TLR3, TLR4, TMEFF1 / Tomoregulin-1, TNF RI / TNFRSF1A, TNF RII, TNF-alpha, TNF-beta / TNFSF1B, TPO, TRAIL / TNFSF10, TRAIL R2 / TNFRSF10B, TRANCE / TNFSF11, TREM-1, TROY, TSLP, TSLP R, TWEAK / TNFSF12, TWEAK R / TNFRSF12, Ubiquitin, uPAR, Urokinase, VCAM-1, VE-Cadherin, VEGF, VEGF R1, VEGF R2, VEGF R3, VEGF-B, VEGF-C, VEGF-D, WIF-1, WISP-1 / CCN4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Detection Buffer C
  • Detection Buffer D
  • Wash Buffer 1
  • Wash Buffer 2
  • HRP-Conjugated Streptavidin Concentrate
  • Plastic Sheets
  • Spin Columns
  • Labeling Buffer
  • Lysis Buffer
  • Plastic Incubation Tray
Other Materials Required
  • Distilled or de-ionized water
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • 2?5 ml tube
  • 15 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Purify Samples with Spin Column
  2. Biotinylate Samples
  3. Purify biotinylated samples using spin column
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Excel (AAM-BLM-1_Analysis_Tool.xls)

citations

  • Prevention of radiotherapy-induced pro-tumorigenic microenvironment by SFK inhibitors

    Year:
    2025
    Journal Name:
    Theranostics
    PubMed ID:
    39776795
  • Matrix-Rigidity Cooperates With Biochemical Cues in M2 Macrophage Activation Through Increased Nuclear Deformation and Chromatin Accessibility

    Year:
    2025
    Journal Name:
    Advanced science (Weinheim, Baden-Württemberg, Germany)
    PubMed ID:
    39828979
  • Tumor-associated macrophages-educated reparative macrophages promote diabetic wound healing

    Species:
    Mouse
    Sample Type:
    tumor-associated macrophages (TAMs)
    Year:
    2022
    Journal Name:
    Embo Molecular Medicine
  • Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma

    Species:
    Mouse
    Sample Type:
    Conditioned Media (RUNX2 KO and with cell lines)
    Year:
    2020
    Journal Name:
    Cancer Cell
    PubMed ID:
    32707078
  • Regulatory role of apelin receptor signaling in migration and differentiation of mouse embryonic stem cell-derived mesoderm cells and mesenchymal stem/stromal cells

    Species:
    Mouse
    Sample Type:
    embryoid bodies (EBs)
    Year:
    2023
    Journal Name:
    Human Cell
  • Analyzing the role of cancer-associated fibroblast activation on macrophage polarization

    Species:
    Human
    Sample Type:
    cancer-associated fibroblasts (CAFs)
    Year:
    2023
    Journal Name:
    Molecular Oncology
  • Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (Muscle Tissue lysate, new method)
    Year:
    2017
    Journal Name:
    Nature Communications
  • Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells

    Species:
    Mouse
    Year:
    2020
  • Loss of p120-catenin induces metastatic progression of breast cancer by inducing anoikis resistance and augmenting growth factor receptor signaling.

    Species:
    Mouse
    Sample Type:
    Conditioned Media (mouse breast cancer model: Trp53?/?;p120-iKD, Ctnnd1?/?;Trp53?/?, and control Trp53?/? cells)
    Year:
    2013
    Journal Name:
    Cancer Research
    PubMed ID:
    23733751
  • Mesenchymal Stem Cells Increase Alveolar Differentiation in Lung Progenitor Organoid Cultures

    Species:
    Mouse
    Year:
    2019

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