Mouse R-spondin-1, RSPO1 ELISA Kit
Referência MBS933642-48
Tamanho : 48-Strip-Wells
Marca : MyBiosource
R-Spondin Homolog (Xenopus Laevis) (RSPO1) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS933642Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RSPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO1 present is bound by the… More details
Product Overview
Product Name
R-spondin homolog (Xenopus laevis) (RSPO1), ELISA Kit
Full Product Name
Mouse R-spondin-1, RSPO1 ELISA Kit
Product Gene Name
RSPO1 elisa kit
Product Synonym Names
Mouse R-spondin-1 (RSPO1) ELISA kit; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO; OTTHUMP00000180612; OTTHUMP00000180613; R-spondin1; roof plate-specific spondin; R-spondin homolog (Xenopus laevis)
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
6.25 pg/ml -400 pg/ml.
Sensitivity
The minimum detectable dose of mouse RSPO1 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse RSPO1. No significant cross-reactivity or interference between mouse RSPO1 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
29,332 Da
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of RSPO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RSPO1 purchase
MBS933642 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the R-spondin homolog (Xenopus laevis) (RSPO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RSPO1. The ELISA analytical biochemical technique of the MBS933642 kit is based on RSPO1 antibody-RSPO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSPO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSPO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for RSPO1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
R-spondin-1
NCBI Official Synonym Full Names
R-spondin homolog (Xenopus laevis)
NCBI Official Symbol
Rspo1
NCBI Official Synonym Symbols
Rspondin; R-spondin
UniProt Protein Name
R-spondin-1
UniProt Synonym Protein Names
Cysteine-rich and single thrombospondin domain-containing protein 3; Cristin-3; mCristin-3; Roof plate-specific spondin-1
Protein Family
R-spondin
UniProt Gene Name
Rspo1
UniProt Synonym Gene Names
Cristin-3; mCristin-3
UniProt Entry Name
RSPO1_MOUSE
NCBI Protein Information
R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3
UniProt Comments for RSPO1
R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Cell development/differentiation
Cellular Component: extracellular space; extracellular region; nucleus
Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding
Biological Process: Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; response to stimulus; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway
Show more Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Cell development/differentiation
Cellular Component: extracellular space; extracellular region; nucleus
Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding
Biological Process: Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; response to stimulus; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with RSPO1 elisa kit
| Reference Product | PubMed Publications |
|---|---|
| Sry elisa kit | >34 publications with RSPO1 and Sry |
| Sox9 elisa kit | >30 publications with RSPO1 and Sox9 |
| Foxl2 elisa kit | >19 publications with RSPO1 and Foxl2 |
| Lrp6 elisa kit | >11 publications with RSPO1 and Lrp6 |
| Dkk1 elisa kit | >10 publications with RSPO1 and Dkk1 |
| Fgf9 elisa kit | >8 publications with RSPO1 and Fgf9 |
| Dmrt1 elisa kit | >7 publications with RSPO1 and Dmrt1 |
| Lrp5 elisa kit | >4 publications with RSPO1 and Lrp5 |
| Stra8 elisa kit | >2 publications with RSPO1 and Stra8 |
Pathways associated with RSPO1 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Disease Pathway antibodies | Disease Pathway Diagram |
| RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies | RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram |
| Signal Transduction Pathway antibodies | Signal Transduction Pathway Diagram |
| Signaling By WNT In Cancer Pathway antibodies | Signaling By WNT In Cancer Pathway Diagram |
| Signaling By Wnt Pathway antibodies | Signaling By Wnt Pathway Diagram |
| TCF Dependent Signaling In Response To WNT Pathway antibodies | TCF Dependent Signaling In Response To WNT Pathway Diagram |
| XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies | XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram |
| Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies | Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram |
| Regulation Of FZD By Ubiquitination Pathway antibodies | Regulation Of FZD By Ubiquitination Pathway Diagram |
Diseases associated with RSPO1 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Urogenital Abnormalities Antibodies | >23 publications with RSPO1 and Urogenital Abnormalities |
| Skin Diseases Antibodies | >5 publications with RSPO1 and Skin Diseases |
| Embryo Loss Antibodies | >3 publications with RSPO1 and Embryo Loss |
| Necrosis Antibodies | >3 publications with RSPO1 and Necrosis |
| Musculoskeletal Abnormalities Antibodies | >2 publications with RSPO1 and Musculoskeletal Abnormalities |
| Adenocarcinoma Antibodies | >2 publications with RSPO1 and Adenocarcinoma |
| Weight Loss Antibodies | >2 publications with RSPO1 and Weight Loss |
| Hyperplasia Antibodies | >2 publications with RSPO1 and Hyperplasia |
| Neoplasms, Experimental Antibodies | >2 publications with RSPO1 and Neoplasms, Experimental |
| Liver Diseases Antibodies | >1 publications with RSPO1 and Liver Diseases |
Organs/Tissues associated with RSPO1 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Testis Antibodies | >22 publications with RSPO1 and Testis |
| Ovary Antibodies | >21 publications with RSPO1 and Ovary |
| Bone Antibodies | >6 publications with RSPO1 and Bone |
| Embryonic Tissue Antibodies | >3 publications with RSPO1 and Embryonic Tissue |
| Pancreas Antibodies | >2 publications with RSPO1 and Pancreas |
| Mammary Gland Antibodies | >1 publications with RSPO1 and Mammary Gland |
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