Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: HeLa
B: HeLa nucleus
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse Cerebellum
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (GTX70220) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (GTX70220) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (GTX70220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.

The data was published in the journal BMC Biochem in 2007. PMID: 17880698

The data was published in the journal PLoS One in 2013.PMID: 23805200

The data was published in the Nat Commun in 2020. PMID: 33257668

The data was published in the journal PLoS Pathog in 2015. PMID: 25927232

The data was published in the journal Biomed Res Int in 2015. PMID: 25722974

The data was published in the journal PLoS One in 2017. PMID: 29045486

The data was published in the journal PLoS One in 2017. PMID: 29045486

The data was published in the journal Sci Rep in 2017. PMID: 28157211

The data was published in the journal Sci Rep in 2017. PMID: 28157211

The data was published in the journal Biomed Res Int in 2015. PMID: 25834829

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

The data was published in the journal J Biomed Sci in 2014. PMID: 25030234

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

The data was published in the journal Nat Commun in 2016.PMID: 27046438

The data was published in the journal Nat Commun in 2016.PMID: 27046438

The data was published in the 2019 in Elife. PMID: 31663849

The data was published in the 2019 in Elife. PMID: 31663849